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Volume 8 Journal of Biotechnology & Biomaterials
ISSN: 2155-952X Adv.Biotech 2018
November 15-17, 2018
November 15-17, 2018 | Berlin, Germany
4th International Conference on
Advances in Biotechnology and Bioscience
Development and characterization of microsatellite markers in Attalea speciosa and their
transferability to six other Attalea species
Lorena Ramos da Mata
Embrapa Genetic Resources and Biotechnology, Brazil
Babassu (Attalea speciosa Mart. ex Spreng) is a native palm tree with wide distribution in Brazil, where it is one of the
main extractive products. The nut extracted from this palm is used in the oil manufacture with domestic and industrial
utility. The sustainable use of babassu is important for the preservation of this forest resource and also for the economy of
endemic regions of the genus. Microsatellite primers were developed for the main species of Attalea. Total genomic DNA
library enriched for TC repeats was constructed for A. speciosa following a standard protocol, and 84 markers were developed.
We evaluated the transferability of the 84 microsatellite markers for six other babassu species: A. barreirensis, A. eichleri, A.
funifera, A. maripa, A. phalerata and A. vitrivir. The transferability was superior to 63% in all species studied. The markers were
characterized in populations of seven Attalea species with 20 to 44 individuals each. The total number of alleles over all loci,
observed and expected heterozygosity was similar for the seven Attalea species, ranging from 6.2 to 9.4, 0.64 to 0.74, and 0.48
to 0.56, respectively. These values are comparable to the values obtained for other palms and showed that the seven Attalea
species has high genetic diversity. Cluster analysis showed groupings of plants according to their species and also showed that
some of the plants are hybrids (A. speciosa x A. eichleri ). The set of markers developed constitutes a powerful tool for genetic
analysis in the genus Attalea.
Biography
Lorena Ramos da Mata has completed her Graduation in Pharmaceutical Sciences at the University of Brasilia in 2008 and MSc in Botany at the same university
in 2016. She is currently an Analyst at the Brazilian Agricultural Research Company-EMBRAPA CENARGEN.
Lorena Ramos da Mata, J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C6-104