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Volume 8 Journal of Biotechnology & Biomaterials
ISSN: 2155-952X Adv.Biotech 2018
November 15-17, 2018
November 15-17, 2018 | Berlin, Germany
4th International Conference on
Advances in Biotechnology and Bioscience
Optimization and evaluation of SSR primers for Urochloa brizantha
Nayara Carvalho1, Felipe Mont`alvão Canela1, Diva Maria de Alencar Dusi2, Lucimara Chiari 2, Vera Tavares de Campos Carneiro3, Souza N O S1 and
Buso G S C2
1University of Brasilia, Brazil
2Embrapa Genetic Resources and Biotechnology, Brazil
3Embrapa Gado de Corte, Brazil
The genus Brachiaria/Urochloa belongs to the family Poaceae, and comprises approximately 100 species. The genetic
characterization represents a great help for the knowledge of genetic resources and conventional breeding. SSR (simple
sequence repeats) molecular markers have been used as an efficient tool for genetic variability analysis. The design of the
primers used in this study is based on the sequences available in the B. brizantha expressed sequence database of the project
"Functional genomics and genetic control of sexual reproduction and apomixis of plants with biotechnological perspectives",
which after mining, clustering and reduction of redundancy, resulted in 315 SSR-EST (expressed sequence tags) primers. Thus,
the objective of this study is to evaluate and characterize the primers for U. brizantha. A total of 40 accessions of the Brachiaria
germplasm active bank of Embrapa Gado de Corte were used. The loci were amplified by PCR reactions (Polymerase Chain
Reaction), the separation of the fragments performed by electrophoresis in 5% polyacrylamide gel and analysis by band
presence or absence. From a total of 154 primers, 81 presented polymorphism representing 52.59% of the total tested. In a
preliminary analysis, four polymorphic SSR primers were used for the 40 accessions that generated a total of 30 markers and
grouped the accessions in a similarity that ranged from 0.18 to 1. Two groups were formed with 0.26 and 0.21 of similarity
respectively. Thus, these primers are promising for the future study of accessions variability of the germplasm bank.
Biography
Nayara Carvalho has completed her Graduation in Agronomy at University of Brasilia (Brazil) in 2015. She is pursuing her PhD at the same university and is working
on molecular markers since graduation. She has published one paper in Genetic and Molecular Research journal.
Nayara Carvalho et al., J Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C6-104