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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Adv.Biotech 2018
November 15-17, 2018
November 15-17, 2018 | Berlin, Germany
4
th
International Conference on
Advances in Biotechnology and Bioscience
An improvedbiofabricationprocess to enhance cell survival of cartilage regenerationand functionality
of the osteoarthritic knee when enriched with bone marrow mesenchymal stem cells
José Manuel Baena, J L Carrillo
and
J A Marchal
1
REGEMAT 3D, Spain
2
IBIMER-University of Granada, Spain
T
issue regeneration (TR) is currently one of the most challenging biotechnology unsolved problems. Tissue engineering
(TE) is a multidisciplinary science that aims at solving the problems of TR. TE could solve pathologies and improve the
quality of life of billions of people around the world suffering from tissue damages. New advances in stem cell (SC) research
for the regeneration of tissue injuries have opened a new promising research field. However, research carried out nowadays
with two-dimensional (2D) cell cultures do not provide the expected results, as 2D cultures do not mimic the 3D structure of a
living tissue. Some of the commonly used polymers for cartilage regeneration are polylactic acid (PLA) and its derivatives such
as poly-L-lactic acid (PLLA), poly (glycolic acids) (PGAs) and its derivatives such as poly (lactic-co-glycolic acids) (PLGAs)
and polycaprolactone (PCL). All these materials can be printed using fused deposition modeling (FDM), a process in which
a heated nozzle melt a thermoplastic filament and deposit it in a surface, drawing the outline and the internal filling of every
layer. All this procedures uses melting temperatures that decrease viability and cell survival. Research groups around the world
are focusing their efforts in finding low temperature printing thermoplastics or restricted geometries that avoid the contact
of the thermoplastic and cells at a higher temperature than the physiologically viable. This has mainly two problems; new
biomaterials need a long procedure of clearance before they can be used in clinical used, and restrictions in geometries will
limit the clinical application of 3D printing in TE.
bd1@regemat3d.comJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C6-104