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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Adv.Biotech 2018
November 15-17, 2018
November 15-17, 2018 | Berlin, Germany
4
th
International Conference on
Advances in Biotechnology and Bioscience
Interaction of chitosan, curcumin, and hydroxyapatite nanoparticles on the reproductive system and
its molecular parameters in male rat
Mokhtar Ibrahim Yousef
1
, Haitham Hassen Abd
1
, Maher Abd El-naby Kamel
2
and
Yaser Mohamed Helmy Elkamary
3
1
Institute of Graduate Studies and Research-Alexandria University, Egypt
2
Medical Research Institute-Alexandria University, Egypt
3
European Egyptian Pharmaceutical Industries, Egypt
H
ydroxyapatite nanoparticles (HAP NPs) have been widely used as a biocompatible ceramic in many areas of medicine,
but mainly for contact with bone tissue, due to its resemblance to mineral bone. Risk assessment of HA NPs has attracted
extensive attention despite their promising potential in many biomedical applications, that’s why the present study aimed to
investigate the toxic effects of HAP NPs on the reproductive system and its molecular parameters in male rat and the protective
role of nano-antioxidants (Chitosan CSNPs and curcumin CurNPs) alleviating this toxicity. The present results indicated that
HAP NPs showed significant decrease in semen characteristics and testosterone level, 17-ketosteroid reductase, antioxidant
enzymes (GST, SOD, CAT, GPx and TAC), and reduced glutathione in testes; Total antioxidant capacity and total oxidative stress
levels were determined to evaluate the oxidative injury, While HAP NPs caused significant increase in LH and FSH, abnormal
sperm, 17β-hydroxysteroid dehydrogenase, TBARs, NO, p53, TNFα and interliukin-6 in testes. The DNA damage was also
analyzed by suppression of the mtTFA, induction of UCP2 gene and 8-OHdG level as indicators of genotoxicity. In conclusion,
the presence of CSNPs and CurNPs with HAP NPs showed obvious improvements in the activities of the antioxidant enzymes
reduced glutathione and improved sperm parameters also, alleviated its reproductive toxicity via its ameliorative effects on the
fertility, testicular tissue functions, antioxidant system and hormonal status.
yousefmokhtar@yahoo.comJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C6-104