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Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Adv.Biotech 2018
November 15-17, 2018
November 15-17, 2018 | Berlin, Germany
4
th
International Conference on
Advances in Biotechnology and Bioscience
Mesenchymal stem cell expansion with small molecules
Lamia Yazgi Alyazici
Yeditepe University, Turkey
M
esenchymal stem cells are present in the adult body; they can self-renew themselves and exhibit multipotency. They can
differentiate to bone, fat, chondrocyte and other various cell types under specific conditions. They are a great candidate
for transplantation-based therapies with their immunomodulatory abilities, differentiation potentials and because of their
easy accessibility. They can be obtained from different tissue types including; bone marrow, adipose tissue, umbilical cord
etc. Isolation of MSCs is easy but there are major challenges on mobilization, expansion, understanding the differentiation
mechanism. If these challenges overcame, MSCs show great potential for experimental and clinical applications. In this study,
author has focused on expansion of mouse bone marrow-MSCs, with small molecule treatment. She has selected the effective
molecules conducting WST-1 assay, pyronin y/hoechst staining, cell cycle analysis, apoptosis analysis and settled on four
different molecules. First and most effective molecule is a GSK-3 inhibitor that stabilizes free cytosolic β-catenin and inhibits
differentiation. Second one is a p38-MAPK inhibitor. The goal after this study is to carry this knowledge to therapeutic field.
yazgialyzc@gmail.comJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C6-104