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Volume 4, Issue 3 (Suppl)

Adv Crop Sci Tech

ISSN: 2329-8863 ACST, an open access journal

Page 78

Notes:

Plant Genomics 2016

July 14-15, 2016

conferenceseries

.com

July 14-15, 2016 Brisbane, Australia

4

th

International Conference on

Plant Genomics

New insights into genome-wide change of DNAmethylation and its association with gene expression in

cadmium-exposed rice (

Oryza sativa

)

Zhi Min Yang

1

, Sheng Jun Feng

1

, Xue Song Liu

1

, Shang Kun Tan

1

, Shan Shan Chu

1

, Hua Tao

1

, Tao Liu

1

and

Youko Oono

2

1

Nanjing Agricultural University, China

2

National Institute of Agrobiological Sciences, Japan

P

lant exposure to cadmium (Cd) affects transcriptional responses. Whether Cd modified DNA methylation marks are associated

with transcription and functional consequences in plants remain unknown. We present the genome-wide single base resolution

maps of methylated cytosines in Cd exposed rice, along with global transcriptional change in mRNA. Widespread differences

were identified in the composition and patterning of CG and non-CG methylation marks between Cd exposed and control rice

genomes. There are 2393 non-redundant differentially methylated regions (DMRs). RNA sequencing revealed that most of DNA

methyltransferases, histone methyltransferases and DNA demethylases differentially changed in transcription under Cd exposure. By

profiling global DNA methylation and gene transcription, we found more genes hypermethylated than those hypomethylated in CG,

CHH and CHG (where H is A, C or T) contexts in the regions of upstream, gene body and downstream under Cd stress. Seventy-nine

genes (p<0.05, two-fold change) with a strong preference of differential expression in Cd exposed rice plants was identified. Many of

themwere involved in stress response, metal transport and transcription factors. A subset of loss of functionmutants defective in DNA

methylation/demethylation and histone modification activities were used to identify transcript abundance of selected genes. In most

cases, genes in the mutants were repressed by Cd treatment. Provision of azacitidine (a global DNA methylation inhibitor) attenuated

root growth inhibition but promoted biomass and Cd accumulation under Cd exposure. Finally, we identified 108 transposons and

254 retrotransposons that were modified by methylation, where transcriptional expressions of 30 neighboring genes were changed

under Cd exposure.

Biography

Zhi Min Yang is a Professor of Plant Physiology and Molecular Biology in Nanjing Agricultural University (NAU). He has earned his PhD degree at NAU in 1999

and received his Postdoctoral training at the University of Kagawa, Japan and Justus-Liebig University, Germany. He is working on physiological and molecular

mechanisms for plant abiotic stress responses (salt, drought or heavy metals). His major interest is in epigenetic regulation of stress tolerance, toxic metal uptake

and accumulation in plants through small/long non-coding RNAs (e.g. microRNAs) and DNA methylation. He has published 80 peer-reviewed papers and serves

as Associate/Academic Editor of

Gene, Plant Gene and PloS One

.

zmyang@njau.edu.cn

Zhi Min Yang et al., Adv Crop Sci Tech 2016, 4:3 (Suppl)

http://dx.doi.org/10.4172/2329-8863.C1.002