euro-infectious-diseases-2016_posters-accepted-abstracts

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Volume 4, Issue 5(Suppl) J Infect Dis Ther
ISSN: 2332-0877 JIDT, an open access journal
Page 43
Euro Infectious Diseases 2016
September 05-06, 2016
conferenceseries.com
Infectious Diseases
September 05-06, 2016 Frankfurt, Germany
3rd Euro-Global Conference on
Assessment of pathogenicity and tissue distribution of infectious bronchitis virus strains (Italy 02 genotype)
isolated from Moroccan broiler chickens
Khadija Khataby1, 2, Faouzi Kichou3 , Chafiqa Loutfi2 and My Mustapha Ennaji1
1University Hassan II Mohammedia, Morocco
2Society Biopharma, Morocco
3Agronomic and Veterinary Institute Hassan II, Morocco
Avian infectious bronchitis (IB) is one of the most important viral diseases of poultry, affecting chickens of all ages and causing
major economic losses in poultry flocks. The aim of this study is to evaluate pathogenicity and tissue distribution of Moroccan
Italy 02 genotype of infectious bronchitis virus (IBV). Total 40 one day old specific pathogen free chickens were divided randomly
into four groups. Group-1, 2 and 3 were inoculated intra oculo-nasally with 103.5 EID50 of virus and Group-4 was kept as control.
Chickens in each group were monitored for 14 days post-infection (dpi). Chickens in all infected groups showed severe respiratory
signs which most of them have been reproduced on 2 dpi with varying times of appearance and disappearance. The infected birds
appeared lethargic, reluctant to move with specific respiratory signs and macroscopic lesions. The specific histological lesions
developed in all infected birds, confirm the ability of the three tested strains to induce severe respiratory disease. The results at 14
dpi also revealed that all strains were able to induce serological response. Virus re-isolation from infected organs and amplification
of the viral RNA by real-time PCR proved the presence of the virus in lung and trachea of infected chicks. Neither re-isolation nor
significant viral RNA detection were detected in the kidney. These results demonstrated that the three strains Italy 02 genotype
emerging in Moroccan poultry farms have a wide distribution for respiratory system without kidney damage and without causing
mortality.
J Infect Dis Ther 2016, 4:5(Suppl)
Ulceroglandular tularemia: Clinical course and outcome in 5 cases
Liliya Markova Pekova1, 2, P Parusheva2, M Fartunova2, N Dimitrov2 and I Tomova3
1Trakia University, Bulgaria
2University Hospital Stara Zagora, Bulgaria
3Ministry of Health, Bulgaria
Introduction: Tularemia is an acute febrile zoonosis, which is potential life threatening disease. The most popular clinical form is
ulceroglandular.
Materials & Methods: In a period of 8 months (December 2014-July 2015) through the Clinic of Infectious diseases of University
Hospital Stara Zagora, Bulgaria, 5 patients with ulceroglandular form of Tularemia were passed; they were 4 men and 1 woman, aged
from 52 to 73 years. Clinical, epidemiological, laboratorial and serological investigations were provided.
Results & Discussion: Three of patients were hunters, fired wild rabbits and the rest two took part in breaking up and cooking
the game. After an incubation period mean 4.8±1.4 days in all was appeared a primary affect in region of the fingers. Three had
redness in eyes, on face and oropharynx. Later on the toxic syndrome a regional painful lymphadenitis was appeared. Two patients
had dyspeptic syndrome with vomiting and diarrhea, no appetite. All had enlarged liver, two had scanty maculopapular rash. The
diagnosis was proved by degree agglutination type Vidal at the Referent Laboratory of Particularly Dangerous Infection, National
Center of Infectious, Parasite Diseases, Sofia. The antibacterial treatment was a combination of 4-quinolones and aminoglycosides.
The outcome was favorable in all patients.
Conclusion: Because tularemia is not so common disease, it could be easy left out. It must be suspected when there were unusual
temperature, redness in eyes and glandular syndrome with corresponding epidemiological situation.