euro-infectious-diseases-2016_posters-accepted-abstracts

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Volume 4, Issue 5(Suppl) J Infect Dis Ther
ISSN: 2332-0877 JIDT, an open access journal
Page 42
Euro Infectious Diseases 2016
September 05-06, 2016
conferenceseries.com
Infectious Diseases
September 05-06, 2016 Frankfurt, Germany
3rd Euro-Global Conference on
Delivery of Mycobacterium tuberculosis lipids using chitosan nanoparticles induce potent cytokine and
antibody response through activation of γδ T-cells in mice
Ishani Das and Avinash Sonawane
1National Institute of Cholera and Enteric Diseases, India
2Translational Health Science and Technology Institute, India
Activation of cell mediated and humoral immune responses to Mycobacterium tuberculosis (Mtb), the causative agent of
tuberculosis (TB), are critical for protection. For decades, most studies regarding immunity to TB are focused mainly on
proteins. In recent years, increasing evidences have shown that Mtb cell wall lipids act as potent adjuvants as well as antigens capable
of activating specific T-cells through their presentation by CD1 molecules and also induce IgM, IgA and IgG antibody responses.
However unlike proteins, delivery and presentation of lipid antigens is a major challenge. Herein, we have used chitosan nanoparticles
(NPs) as Mtb lipid delivery system and showed that chitosan NP mediated delivery of Mtb lipids induce potent cytokine and antibody
responses in immunized mice. Chitosan NP delivered Mtb lipids induced the release of most prominent cytokines associated with
Th1 (TNF-α, IFN-γ, IL-2) and Th2-type (IL-4, IL-5, IL-6, IL-13) immune responses in mice lymphatic and spleen cells as compared
to immunogenic Mtb purified protein derivative (PPD) and chitosan NPs alone. Moreover, mice immunized with Mtb lipid coated
chitosan NPs showed significantly higher levels of IgG, IgG1 and IgM and a moderate increase in IgG2a antibodies as compared to
Mtb lipid liposomes and chitosan NP immunized mice. In conclusion, this study represents a promising new strategy for efficient
delivery of Mtb lipids to trigger enhanced cell mediated and antibody response against Mtb infection.
J Infect Dis Ther 2016, 4:5(Suppl)
Questionnaire triagingmodel adopted in rapid screening patients who are suspected to have Ebola infection
Jin Li, Tao Yan, Jinsong Mu, Enqiang Qin, Ye Wang, Liying Liu and Dan Wu
302 Military Hospital, China
Introduction & Aim: Ebola disease (EVD) is a fetal viral infectious disease with case fatality as high as 90%. Early discriminating
patients on admission into high risk or low risk are of extremely important, thus the patients can be delivered to corresponding
isolation areas to avoid Ebola viral cross transmission. However, diagnostic lab results of patients are difficultly obtained at first,
especially during the early period of outbreak. A questionnaire triaging model was designed and then applied in a holding center in
Sierra Leone successfully during the outbreak 2014.
Methodology: Medical histories of the patients were collected on admission according to case investigation form made by ‘WHO’,
including 21 symptoms. The patients were diagnosed to have or not to have EVD 2 to 3 days after admission based on results of RT-
PCR. Symptoms between the EVD and non-EVD patients were analyzed and compared. A criterion of screening the patients was
made based on the results of date analysis.
Results: The total symptoms of EVD patients were significantly higher than those of non-EVD patients (9 vs. 5.5; p<0.001). Cut-
off value was 6, the sensitivity, specificity, positive predictive value and negative predictive value were 70.4, 76.1, 87.4 and 52.2 %
respectively. We assigned suspected patients to high risk area if the amount of their symptoms were equal or greater than 6; or to
low risk area if less than 6. Patients were separated by space in both quarantine areas according to the Sierra Leone Emergency
Management Program Standard Operating Procedures for Managing Ebola Virus Disease in Holding Centers. By the end of our work
in Sierra Leone, there was no patient of cross transmission reported under this triaging model.
Conclusions: Questionnaire triaging model is efficient in screening suspected EVD patients in highly epidemic regions, which could
be reference in patient management for other fetal infectious diseases. Even though clinical manifestations of EVD patients are
generally non-specific, the amount of all symptoms could be of more diagnostic value.