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conferenceseries
.com
Volume 05
Neonatal and Pediatric Medicine
ISSN: 2572-4983
World Pediatrics 2019
December 04-05, 2019
December 04-05, 2019 | Barcelona, Spain
32
nd
World Pediatrics Conference
Exploration of the FLT3-ITD mutation in acute myeloid leukemia patients in the western Algerian
population
Wefa Boughrara
Higher School of Biological Sciences of Oran, Algeria
Statement of the Problem
: Leukemia affects 9,000 people worldwide each year; with 3/700 have acute myeloid leukemia
(AML).They arise frommutations that affect the genes influencing hematopoiesis. FMS-related tyrosine kinase 3 (FLT3) is
a tyrosine kinase receptor usually expressed in hematopoietic progenitors, is themost common genetic lesion inAMLwith
mutations detected in 25% to 40% of cases. There are two main types of mutations: tandem internal duplication (ITD),
which is the most common (~ 25% of cases) and a point mutation D835 (TKD) (~ 5%). The detection of FLT3-ITD is
important for the prognosis especially in those who have a normal karyotype.
Aim
: The aim of study is evaluate the FLT3-ITDmutation frequency in the western Algeria population.
Material and Methods
: We analyzed eighty-one patients with cytogenetic and molecular biology department at the
University Hospital of Oran (EHU) and those from March 2014 to March 2018. We explored the FLT3- ITD mutation
using the polymerase chain reaction (PCR).
Results
: Statistical analysis showed that out of eighty-oneAML patients, only eleven cases had the FLT3-ITDmutationwith
the heterozygous state. Which corresponds to a frequency of 12%?These results are in perfect agreement with the Chinese
population estimated at 11%. However, our results are in disagreement with those reported in European population (50%)
and the Egyptian population of 34.6%.
Significances
: In this study, we highlighted the frequency of the FLT3-ITD mutation in the western Algerian population.
It would be very interesting to consider undertaking a study on the impact of the size of the FLT3-ITD fragment on the
prognosis since a study has shown that duplications of 48 to 60 base pairs are associated with very poor prognosis.
Neonat Pediatr Med 2019, Volume: 05
FLT3-ITD normal/mutation version