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Page 89
Volume 7, Issue 6 (Suppl)
J Biotechnol Biomater, an open access journal
ISSN: 2155-952X
World Biotechnology 2017
December 04-05, 2017
2
nd
World Biotechnology Congress
December 04-05, 2017 | Sao Paulo, Brazil
Effect of
Melissa officinalis
on rat hippocampal and cortex slices subjected to oxygenandglucose deprivation
Mushtaq Ahmed
UST-Bannu, Pakistan
C
erebral stroke is 3
rd
leading cause of death after cancer and heart attack worldwide. Free radicals play key role in brain injury.
The results of the present investigation demonstrate that Oxygen-glucose deprivation (OGD) followed by re-oxygenation led
to cell damage/death via an increase in free radical’s production in rat brain hippocampal and cortex slices compared with control
(non-OGD) after 2h OGD followed by 1h reperfusion.
Melissa officinalis
at concentration 40 µg/ml displayed potential role in
neuro-protection against OGD, followed by re-oxygenation in mitochondrial viability assays
in vitro
. In addition,
Melissa officinalis
decreased or slowdown the production free radical in the supernatant and slices homogenate of hippocampal and cortex at the end
of 2h OGD followed by 1h reperfusion. Furthermore, higher concentrations of
Melissa officinalis
slightly showed neurotoxicity for
hippocampal and cortex slices which could be due to a pro-oxidant effect.
mushtaq213@yahoo.comJ Biotechnol Biomater 2017, 7:6 (Suppl)
DOI: 10.4172/2155-952X-C1-086