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Volume 09
February 15-16, 2019 Amsterdam| Netherlands
Vascular Dementia
11
th
International Conference on
Vascular Dementia 2019
Journal of Alzheimers Disease & Parkinsonism | ISSN : 2161-0460
Association of IL-6 and TNF-α gene polymorphisms with the risk of
Alzheimer’s disease in Saudi subjects
A
lzheimer’s disease (AD) is a progressive neurodegenerative disorder and the most
prevalent type of dementia. In Saudi Arabia although the exact percent about the
spread of AD has not been estimated, but the experts believe that there are approximately
50,000 patients in Saudi Arabia, most of them being females. Many of the studies illustrate
the role of the inflammation in development of AD, however no such study has been
done on Saudi AD patients. Thus, the aim of this study was to investigate the association
of inflammatory mediator’s, interleukin-6 (IL-6), Tumor necrosis factor-α (TNF-α) and
C-reactive protein (CRP) with increased the risk of Alzheimer’s disease (AD). Further, the
association between the level of IL-6, TNF-α and CRP with the genetic variation in IL-6
(-174 rs1800795 G/C and -572 rs1800796 C/G), and in TNF-α (-308 rs1800629 A/G and
-1031 rs1799724 C/T) and their role in occurrence ofAD in among Saudi ethnic population
was investigated. A total of 47 Saudi subjects with age (65-90 years) were enrolled for
the study, 24 (14 male, 10 female) diagnosed as AD patients and 23 (11 male, 12 female)
served as normal controls. The level of biomarkers (IL-6, TNF-α and CRP) were assessed
by ELISA (Quntikine®ELISA). Single nucleotide polymorphism (SNP) in selected genes
was analyzed by RT-PCR using TaqMan Assay. This study showed that TNF-α was higher
in AD patients with CC and GC genotypes for IL-6 gene SNPs rs1800796 (P=0.062) and
rs1800795 (P=0.066) respectively. The level of IL-6 was also found to be significantly low
among AD patients with AG genotype comparing to AD patients with GG genotype for
-308 A/G (rs1800629) of TNF-α gene (P=0.040). In conclusion; the level of inflammatory
cytokines IL-6 and TNF-α may play role in the progression of AD depending on specific
genotypes among Saudi AD patients.
Biography
Reem Almutairi currently master student in biochemistry department, collage of science, King Saud University,
riyadh, Saudi Arabia. I have got the license of health professional classification under the name (laboratory
technician). from saudi commission for healthspecialties.I am working as volunteer researcher assistant in
biochemistry department
reemmoteb@gmail.comReem Almotairi, J Alzheimers Dis Parkinsonism 2019, Volume 09
DOI: 10.4172/2161-0460-C1-060
Reem Almotairi
King Saud University,
Saudi Arabia