12 / 23
Notes:
Volume 7
Traditional and Restorative Medicine & Neuropharmacology 2018 | August 27-29, 2018
Journal of Traditional Medicine & Clinical Naturopathy | ISSN : 2573-4555
conferenceseries LLC Ltd
JOINT EVENT
Global Summit on
Traditional & Restorative Medicine
10
th
World Congress on
Neuropharmacology
Page 33
August 27-29, 2018 | Paris, France
Electroacupuncture attenuates visceral hypersensitivity by inhibiting JAK2/
STAT3 signaling pathway in the descending pain modulation system
Background & Aim:
Electroacupuncture (EA) has been used for treating visceral
hypersensitivity (VH). However, the underlying molecular mechanism remains unclear.
The aim of this study was to testify the effect of EA on ileitis-provoked VH, and to
confirm whether EA attenuates VH through Janus kinase 2 (JAK2)/ signal transducers
and activators of transcription 3 (STAT3) signaling pathway in the descending pain
modulation system.
Methods:
Goats were anaesthetized for injecting 2,4,6-trinitro-benzene-sulfonic acid
(TNBS)-ethanol solution into the ileal wall to induce VH. EAwas treated for 30 min from
day 7, then every three days for six times. VH was assessed by visceromotor response
(VMR) and pain behavior response to 20, 40, 60, 80 and 100 mmHg colorectal distension
pressures at day 7, 10, 13, 16, 19 and 22. The spinal cord in the eleventh thoracic vertebra
and brain were collected at day 22. The protein and mRNA levels of IL-6, JAK2 and
STAT3 in SCDH were detected. The distribution of these substances was observed in
ventrolateral PAG (vlPAG), RVM (mainly the nucleus raphe magnus, NRM), SCDH,
nucleus tractus solitaries (NTS) and dorsal motor nucleus of vagi (DMV).
Results:
Goats administered with TNBS-ethanol showed enhanced VMR and pain
behavior response, and increased IL-6, phosphorylated JAK2 and STAT3 (pJAK2 and
pSTAT3) in vlPAG, NRM, NTS and DMV, and their protein and mRNA levels in the
SCDH. EA relieved VMR and pain behavior response, decreased IL-6, pJAK2 and
pSTAT3 levels in vlPAG, NRM, SCDH, NTS.
Conclusion:
EA attenuates VH probably through inhibiting JAK2/STAT3 pathway in the
PAG-RVM-SCDH axis.
Biography
Juan Wan is pursuing her PhD in Huazhong Agricultural University. Her major subject is Clinical Veternary. Her
research focus on acupuncture analgesia. She has published three papers in reputed journals.
wanjuanyimi@163.comJuan Wan, J Tradit Med Clin Natur 2018, Volume:7
DOI: 10.4172/2573-4555-C1-001
Juan Wan
Huazhong Agricultural University, China