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Volume 5 Toxicology: Open Access Toxicology Congress 2019
May 06-07, 2019
May 06-07, 2019 Tokyo, Japan
20th World Congress on
Toxicology and Pharmacology
Rishikesh Kumar Tiwari et al., Toxicol Open Access 2019, Volume 5
DOI: 10.4172/2476-2067-C1-008
Toxicological evaluation of chlorpyrifos, cypermethrin and their combination in earthworm and
their impact on acetylcholinesterase
Rishikesh Kumar Tiwari, Shikha Singh and Ravi S Pandey
University of Allahabad, India
The increasing applications of pesticides in the agricultural fields have adverse impact on flora and fauna of the soil
ecosystem. The role of earthworms in the agricultural practices is well known as they immensely contribute in increasing
the quality and fertility of soil. So, it acts as a bio-indicator for the ecotoxicological analysis of pesticide induced soil pollution.
Therefore, the present study was aimed to explore the impact of chlorpyrifos (an organophosphate; OP), cypermethrin (a
pyrethroid) and their combination (chlorpyrifos+cypermethrin) on earthworm, Eudrilus eugeniae. E. eugeniae were exposed
to different concentrations of pesticides for 48 hours by paper contact toxicity method. The LC50 for commercial grade
chlorpyrifos, cypermethrin and their combination were determined as 0.165, 0.020 and 0.066 μg/cm2 respectively. To assess
the sub-lethal effect of these pesticides, E. eugeniae were exposed to 5% and 10% of LC50 pesticides for 48 hours. Alterations
in morpho-behavioral patterns such as coiling, clitellar swelling, mucus release and bleeding followed by fragmentation
of body in earthworms were observed following exposure. Acetylcholinesterase (AChE) activity was assayed in different
regions of body segment which exhibits significant (p<0.05) decrease in AChE activity particularly in pre-clitellar region
followed by clitellar and post-clitellar regions and in comparison, to whole body. The decreased AChE activity with increasing
concentration of pesticides indicates the effect at neuronal level which apparent from the behavioral changes. Therefore, from
the present findings it can be concluded that long term exposure to these pesticides could lead to severe and irreparable effects
on biochemical mechanisms of earthworms.
Biography
Rishikesh Kumar Tiwari is presently working as a Doctoral Fellow with Professor Ravi S Pandey, Biochemistry Laboratory, Department of Zoology, University
of Allahabad. He has completed his MSc with Cytogenetics specialization from University of Allahabad, India. He has 10 research/review articles in national/
international journal of repute and one book chapter in USA based publishing house.