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Volume 5

Toxicology: Open Access

Toxicology Congress 2019

May 06-07, 2019

May 06-07, 2019 Tokyo, Japan

World Congress on

Toxicology and Pharmacology

Tejaswi Chavan et al., Toxicol Open Access 2019, Volume 5

DOI: 10.4172/2476-2067-C1-008

Hepatoprotective effects of omega-3 fatty acids through the modulation of genes involved in lipid

metabolism and inflammatory pathway in alcohol induced hepatotoxicity

Tejaswi Chavan, Abhijit Ghadge, Manjiri Karandikar, Vijaya Pandit, Aniket Kuvalekar

USA

mega-3 fatty acids offer several health benefits. In present investigation, hepatoprotective potential of omega-3 fatty

acids in the form of flax oil and fish oil was assessed against repeated alcohol dosing in male albino Wistar rats. Hepatic

injury was induced by administering 30 % alcohol (1ml/100g b.w./day, p.o). Flax oil and fish oil (500mg/kg b.w./day, p.o)

were administered to hepatotoxicity induced rats. Biochemical parameters were analyzed from serum and liver tissue. The

expressions of fatty acid binding protein 1 (FABP1), peroxisome proliferator activated receptor gamma (PPARγ), sterol

regulatory element binding protein 1 (SREBP1), nuclear factor kappa β (NF-kβ) and tumor necrosis factor alpha (TNF-α)

genes from liver were assayed by semi-quantitative polymerase chain reaction. Administration of flax oil or fish oil prevented

hepatic damage with marked improvement in hepatic function and normalization of lipid profiles in serum and liver. These

interventions normalized oxidative stress through improvements in levels of anti-oxidant enzymes and oxidative stress markers.

Expression of genes such as FABP1, PPARγ were downregulated and SREBP1, NF-kβ and TNF-α were upregulated in alcohol

induced hepatotoxic rats while treatment with flax oil and fish oil showed improvement in these gene expression. Histological

analysis showed normal hepatic architecture in flax oil and fish oil treated animals. The flax oil was found could protect the liver

against alcohol-induced liver toxicity and oxidative stress. However, further clinical studies are required to assess the safety and

benefits of flax oil in human beings.