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Volume 5
Toxicology: Open Access
Toxicology Congress 2019
May 06-07, 2019
May 06-07, 2019 Tokyo, Japan
20
th
World Congress on
Toxicology and Pharmacology
Katarzyna Miranowicz-Dzierżawska et al., Toxicol Open Access 2019, Volume 5
DOI: 10.4172/2476-2067-C1-008
Differences between cytotoxicity of preservatives used in cosmetic industry determined on
consecutive passages of senescent diploid human lung fibroblasts
in vitro
Katarzyna Miranowicz-Dzierżawska, Lidia Zapor, Jolanta Skowroń and Lilianna Marciniak
Central Institute for Labour Protection, Poland
Introduction & Aim:
Cell culture system could be a useful model for aging-related changes. The aim of the study was to assess
whether there are differences between the results of determination of preservatives cytotoxicity obtained on senescent cells in
different age.
Method:
Experiments were conducted to determine the cytotoxicity of four preservatives: Methylparaben, propylparaben,
2-phenoxyethanol and benzalkonium chloride on subsequent passages of senescent human lung CCD-8Lu (ATCC
Ò
CCL-
201
TM
) fibroblasts. The tests were carried out in passages no. 10/18. Xenobiotics cytotoxicity was evaluated using two cell
viability assays: MTT assay, determining metabolic activity of cells and NRU assay, assessing the integrity of cell membranes.
The IC
50
values were used as the main measure for comparing the cytotoxicity of tested compounds.
Results:
The results showed that the preservatives can be ranked according to the increasing cytotoxic potency towards the
tested human diploid lung fibroblasts: 2-phenoxyethanol<methylparaben<propylparaben<benzalkonium chloride. Older cells
became less susceptible then the younger ones with cytotoxic effects of the xenobiotics tested in the majority of cases.
Conclusion:
The passage number of diploid human lung fibroblasts had an important impact on the susceptibility of cells to
preservatives. The test of the integrity of cell membranes (NRU) seems to be more appropriate to assess the cytotoxic effect of
the investigated preservatives on diploid fibroblasts in different ages, which may be related to the mechanism of action of these
compounds.
Biography
Katarzyna Miranowicz-Dzierżawska is currently working in Laboratory of Toxicology, a part of the Department of Chemical, Aerosol and Biological Hazards in the
Central Institute for Labour Protection, National Research Institute. She has completed her graduation from Faculty of Pharmacy with Pharmaceutical Analysis
specialization of the Medical University of Warsaw and PhD from Jagiellonian University, Cracow.
kamir@ciop.pl