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Volume 4 Toxicology: Open Access
ISSN: 2476-2067 Toxicology Congress 2018
March 12-14, 2018
Page 36
conferenceseries.com
March 12-14, 2018 Singapore
14th World Congress on
Toxicology and Pharmacology
Profiling of lipid mediators and fatty acids in metastatic breast cancer in mice
Statement of the Problem: Breast cancer is the most frequently diagnosed tumor in women worldwide and a leading cause
of cancer death. Due to the high incidence rate of breast cancer, the development of screening method is urgently needed.
Targeted lipidomic analysis has indicated the potential of using bioactive lipids and fatty acids as breast cancer biomarkers.
Methodology &Theoretical Orientation: Lipid profiling in plasma was analyzed using GC/MS/MS system based on targeted
lipidomic platform with the assessment of lung metastases progression in mice model (4T1) of breast cancer. To characterize
lipid profile in plasma in the early and late stage of metastasis we focus on bioactive lipids and saturated, monounsaturated and
polyunsaturated fatty acids pathway.
Findings: Based on primary tumor growth and lung metastases, 1-2 weeks period after 4T1 cancer cells inoculation was
defined as early metastatic stage, while 3-4 weeks period after 4T1 cancer cells inoculation as late metastatic stage. The early and
late phase of metastasis was characterized by a different pattern of lipids profile in plasma. The main changes in plasma lipids
profile were observed at the late phase of metastasis evidenced by the reduction of the ratio of polyunsaturated to saturated
fatty acids. Decreased plasma concentration of free fatty acids has been found to be associated with tumor progression in mice.
Conclusion & Significance: The early and late phase of metastasis in murine 4T1 metastatic breast cancer was associated with
plasma lipidome remodeling and activation of lipid signaling mediators coincided with metastasis progression. Identification
of lipid targets that play a role in breast cancer invasion may advance our understanding of the rapid progression of cancer and
may lead to the development of new biomarkers for the disease.
References
1. ChiuHH(2015) An efficient and robust fatty acid profilingmethod for plasmametabolomic studies by gas chromatography-
mass spectrometry. Clin Chim Acta; 7: 183-190.
2. Guo S (2014) Significantly increased monounsaturated lipids relative to polyunsaturated lipids in six types of cancer
microenvironment are observed by mass spectrometry imaging. Sci Rep; 5(4): 5959-5970.
3. Pereira DM (2014) GC-MS lipidomic profiling of the echinodermMarthasterias glacialis and screening for activity against
human cancer and non-cancer cell lines. Comb Chem High Throughput Screen; 17: 450-457.
4. Hilvo M (2014) Monounsaturated fatty acids in serum triacylglycerols are associated with response to neoadjuvant
chemotherapy in breast cancer patients. Int J Cancer; 7: 1725-33.
5. Flowers M (2010) Pilot study on the effects of dietary conjugated linoleic acid on tumorigenesis and gene expression in
PyMT transgenic mice. Carcinogenesis; 9: 1642-1649.
Biography
Maria Walczak has completed her Graduation from the Faculty of Pharmacy Medical Academy in Krakow, Poland and PhD degree from the Faculty of Pharmacy,
Jagiellonian University Medical College (UJ CM) and Habilitation thesis in Pharmacokinetics in 2014. Since 2001 she has been working at the Department of
Pharmacokinetics and Physical Pharmacy UJ CM as a Lecturer, since 2010 at the Jagiellonian Centre for Experimental Therapeutics (JCET) as a Manager of the
Laboratory of Analytics and Pharmacokinetics and since 2015 as a Head of Chair and Department of Toxicology, Faculty of Pharmacy UJ CM. Her scientific work
refers to pharmacokinetic and toxico-kinetic profiling, metabolite screening, targeted lipidomics and proteomics and ligands binding to blood proteins.
Maria Walczak
Jagiellonian University Medical College, Poland
Maria Walczak, Toxicol Open Access 2018, Volume 4
DOI: 10.4172/2476-2067-C1-004