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Volume 8, Issue 1 (Suppl) J Cell Sci Ther
ISSN: 2157-7013 JCEST, an open access journal Stem Cell Research 2017
March 20-22, 2017
March 20-22, 2017 Orlando, USA
8th World Congress and Expo on
Cell & Stem Cell Research
Alfatih A A Aboagla et al., J Cell Sci Ther 2017, 8:1 (Suppl)
LysophosphatidicAcidAcyltransferase2 (LPAT2) enhances abscisic acid response and plays a positive role
in osmotic stress in rice
Alfatih A A Aboagla and Hong Yueyun
Huazhong Agricultural University, China
Lysophosphatidyl acyltransferase (LPAT) is a pivotal enzyme controlling the metabolic flow of lysophosphatidic acid into different
phosphatidic acids in diverse tissues. Recent results begin to shed light onto the involvement of LPAT2 in response to ABA, water
deficits, and salinity. We examined and characterized putative LPAT2 gene in rice (Oryza sativa). LPAT2 transcript is existing in all
tissues tested with relatively higher in leaves and roots, and was induced by salt, drought and ABA treatment, suggesting its roles in
plant growth and stress response. Moreover, LPAT2 is localized to the endoplasmic reticulum (ER) membrane, implicati-ng its role
in lipid metabolism and signaling. Additionally, LPAT2 might be essential for gametophyte or embryo development as homozygous
mutant for a T-DNA insertion in LPAT2 coding region fails to recover in rice. Using a knockdown mutant lpat2 and its genetic
complementation revealed that LPAT2 is important for plants to adapt osmotic stress. Reduced LPAT2 conferred plants and seeds
were more sensitive to ABA treatment and were less tolerant to salt and drought stress. The results suggest that LPAT2 plays a positive
role in ABA response and osmotic stress tolerance. The role of LPAT2 in osmotic stress is mediated by ABA response as shown that
the lpat2 mutant exhibited more water loss from leaves when supplied ABA under salt stress. The ABA responsive gene RAB18 was
less induced by ABA and salt treatments in the lpat2 mutant. The result suggests that LPAT2 enhance ABA response to promote plants
osmotic stress tolerance. PA produced by LPAT2 activity might be also important for ABA response. PA supplementation is capable
of restoring ABA sensing and salt stress tolerance as WT performance. The effects of LPAT2 on plant stress tolerance might be dual
effects of PA, enhanced ABA response and enhanced growth. Our study reveals novel interactions among ABA, LPAT2 and PA and
provides insight into progresses in agronomic traits and adaptive growth through the manipulation of these pathways in rice.
Biography
AlfatihAAAboagla has completed his MSc fromHuazhongAgricultural University, Wuhan, P. R. China. Currently, he is doing his PhD in Microbiology (Bioengineering).