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Volume 8, Issue 1 (Suppl) J Cell Sci Ther
ISSN: 2157-7013 JCEST, an open access journal Stem Cell Research 2017
March 20-22, 2017
March 20-22, 2017 Orlando, USA
8th World Congress and Expo on
Cell & Stem Cell Research
Irina V Panyutin et al., J Cell Sci Ther 2017, 8:1 (Suppl)
Effect of ionizing radiation on the proliferation of human embryonic stem cells
Irina V Panyutin, Sonia A Holar, Ronald D Neumann and Igor G Panyutin
National Institutes of Health, USA
We analyzed growth rates of seven hESC lines by measuring area of individual colonies. The doubling time averaged over all the
colonies varies from 18.9 to 28.7 hours. We studied effect of 0.2 and 1.0Gy exposure on proliferation of these hESC lines. All
cell lines showed similar reaction to IR, i.e. the number of cells dropped within 24-48 h; after that they recover and grow with the
same rate as the sham-irradiated cells. The Relative Cell Survival (RCS) i.e. the fraction of cells in the irradiated samples relative to the
sham-irradiated cells varied from 0.6 to 0.8 after 0.2Gy and from 0.1 to 0.2 after 1Gy IR. The RCS correlates directly with the doubling
time, i. e. the faster cells grow the more radiosensitive they are. The doubling times and areas of individual colonies varies significantly
for all cell lines. For all cell lines except WA22 we found no correlation between colony size and growth rate; however for several cell
lines (H1, WA13, WA19) smaller colonies were more radiosensitive than the larger ones.
Biography
Irina V Panyutin has earned her Doctoral degree in Epidemiology from Moscow State Medical Academy in 1980 and has worked as an Epidemiologist at a Disease
Control Station in Moscow, Russia. After coming to USA, she worked as a Research Scientist at Braton Biotech Inc., Rockville, Maryland from 1992 to 1996. Since
1996, she has been with the NIH, Nuclear Medicine Department. Her research interests include study of the effect of ionizing radiation on human embryonic stem
cells survival, proliferation and differentiation.