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Volume 4, Issue 7(Suppl) J Infect Dis Ther 2016
ISSN: 2332-0877, JIDT an open access journal
Page 45
Notes:
Skin Diseases & Microbiology 2016
October 03-05, 2016
conferenceseries.com
October 03-05, 2016 Vancouver, Canada
International Conference on
Infectious Diseases, Diagnostic Microbiology &
Dermatologists Summit on Skin Infections
GeneXpert: A new tool for the rapid detection of Rifampicin resistance in Mycobacterium tuberculosis
Muhammad Saeed
Allama Iqbal Medical College, Pakistan
To evaluate the diagnostic sensitivity and specificity of GeneXpert (MTB/RIF) assay for the detection of rifampicin (RIF) resistance
in Mycobacterium tuberculosis (MTB) by comparing the results with conventional drug susceptibility testing (DST) as “Gold
Standard”. A total of 2200 pulmonary and extra-pulmonary specimens were collected from TB suspects from 2012 to 2014. All
specimens were processed for ZN staining, LJ culture according to WHO protocol GeneXpert (MTB/RIF) as per manufacturer
instructions. All cases positive for MTB were further processed for DST for RIF. Out of 2200 Tb suspects, 840 (38.18%) cases were
GeneXpert (MTB/RIF) positive for MTB. Among these 15.6% (134/840) cases showed RIF resistance. The sensitivity, specificity, PPV
and NPV of GeneXpert for RIF resistance were found to be 98.3%. 99.1%, 94.7% and 99.4% respectively by comparing the results
with DST. Our study revealed that GeneXpert (MTB/RIF) is an extremely helpful diagnostic tool for detection of RIF resistance in TB
suspects with fairly high sensitivity and specificity along with 2 hours turnout time, which facilitates proper in time management and
treatment among MDR-TB patients in developing countries.
Biography
Muhammad Saeed has completed his BSc in Medical Laboratory Technology from University of Health Sciences Lahore, Pakistan. He is working as a Medical Laboratory
Technologist in Punjab Institute of Cardiology Lahore. He has published more than 9 research papers in well reputed Pakistani journal.
Muhammad Saeed, J Infect Dis Ther 2016, 4:7(Suppl)