skin-diseases-and-microbiology-2016_posters-abstracts

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Volume 4, Issue 7(Suppl) J Infect Dis Ther 2016
ISSN: 2332-0877, JIDT an open access journal
Page 59
Skin Diseases & Microbiology 2016
October 03-05, 2016
conferenceseries.com
October 03-05, 2016 Vancouver, Canada
International Conference on
Infectious Diseases, Diagnostic Microbiology &
Dermatologists Summit on Skin Infections
Prevalence of hepatitis C virus genotype 3 at Civil Hospital, Karachi, Pakistan
Ghulam Fatima1, Suresh Kumar 1, M Saeed Quraishy1 and Shahana Urooj Kazmi2
1Civil Hospital, Pakistan
2Dadabhoy Institute of Higher Education, Pakistan
Aim: This study was designed to find out the frequency of various HCV genotypes present in patients with liver disorders in Karachi,
Pakistan.
Materials &Methods: All patients including, injectable drug users (IDUs), recycled syringe users, those who have undergone invasive
procedures for different ailments, visiting hepatitis clinic at Civil Hospital Karachi, Pakistan, who were positive for hepatitis C virus by
PCR, were screened for the genotyping of hepatitis C virus. Blood samples were collected from the patients in yellow top vacutainers
and allowed to clot, then centrifuged and serum was separated and saved at -400C till further testing. RNA extraction was done with
Promega Kit and HCV genotyping was done on m2000 rt Abbott, using HCV genotyping Amplification Kit.
Results: In order to know the prevalence of hepatitis C virus genotype in our community, we determined HCV genotype for 951
patients, who were positive for HCV RNA, by PCR. It was observed that the most prevalent HCV genotype was “3” detected in
713 (75%) patients, followed by 1a in 63 (6.6%) cases. Genotype 3 affecting all age groups was observed. Females were affected by
genotype 3 than males.
Conclusions: High prevalence of HCV genotype 3 strain among IDUs due to use of recycled syringes and unsafe blood transfusion is a
cause of concern for public health professionals in Pakistan; however timely diagnosis may reduce the chances of serious complications
due to comparatively effective therapeutic response to available antiviral treatment. Our observations call for developing effective
control of factors contributing to high incidence of disease.
Oligonucleotides library production for isolation of aptamers to detect E. coli O157:H7
Mana Oloomi, Saeid Bouzari, Masoum Amraee and Afsaneh Yavari
Pasteur Institute of Iran, Iran
Diarrhea can cause major child mortality in developing countries. E. coli O157:H7 is one of the most important serotypes of
enterohemorrhagic Escherichia coli (EHEC) that can cause diarrhea. It is transmitted to humans through food, and creates
complications such as uremic hemorrhagic colitis. Currently, the standard method for the detection of E. coli O157:H7 is culture
and detection by serology. Recognition by these methods takes more than 36 hours. Thus, access to a test that could detect E. coli
O157:H7 in less time is valuable. The aptamers are the oligonucleotides and short single-stranded DNA or RNA or specific proteins
that have the ability to specifically bind to target. In this regard, aptamer is used, capable of binding tightly and specifically to target
with complex multimeric structures. In this study, a DNA aptamer that can detect E. coli O157:H7 from other similar species was
constructed by Cell-SELEX (Systematic Evolution of Ligands by Exponential Enrichment). A library of DNA aptamer was made.
Streptavidin coated magnetic beads were used to select specific aptamer. Selected aptamers were amplified by PCR, in each step,
then cloned and sequenced. A 117 bp aptamer was selected by six rounds of SELEX method. The aptamer specific binding to E. coli
O157:H7 was also calculated by flow cytometry. Using the new aptamer specific molecular probes may be quick and easy to diagnose
clinically used E. coli O157:H7 bacterial infection. On the other hand, the present method is simple and cost effective for specific
bacterial detection.
J Infect Dis Ther 2016, 4:7(Suppl)