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Volume 7
OMICS Journal of Radiology
Radiology and Oncology 2018
July 16-17, 2018
July 16-17, 2018 Dubai, UAE
Radiology and Oncology
2
nd
World Congress on
New ethanol method for soft tissue imaging in micro-CT
Matej Patzelt
1
, Jana Mrzilkova
1
, Petr Zach
1
, Vladimir Musil
1
, Jakub Karch
2
, Frantisek Krejci
2
, Jan Zemlicka
2
, Jan Dudak
2
and Jozef Rosina
1,2
1
Charles University, Czech Republic
2
Czech Technical University in Prague, Czech Republic
Introduction & Aim:
Micro Computed Tomography (micro-CT) uses X-rays to create sections of examined structures, which can be
afterwards reconstructed into 3D models. Imaging of native soft tissues is very problematic due to their low intrinsic contrast. In the
past years several techniques for attenuation of contrast of soft tissues were developed. It is possible to use either intravascular contrast
agents or fixative agents. Most of these techniques are however quite expensive, very difficult to use or even toxic. The goal of our study
was to find a new fixation method for soft tissue imaging in micro-CT, which would be cheap and easy to use.
Method:
We used mouse organs-heart, lungs, kidneys, liver and brain. We fixed these organs in 97%, 50% ethanol solution and in a
row of ascending concentrations of ethanol (25-97%). We scanned all specimens in micro-CT MARS (Medipix All Resolution System)
after 72 hours, 168 hours and 336 hours period of fixation.
Result:
Ethanol fixation provided contrast enhancement in all studied organs. Fixation in 97% ethanol provided very fast fixation
and the contrast among the tissues was visible already after 72 hours of fixation. Fixation for period of 336 hours gave better detailed
visualization. On the other hand, this type of fixation caused organs to petrify. Fixation in 50% ethanol provided best results after 336
hours of fixation; details were not visualized as good as in 97% ethanol, samples stayed soft. Best results were obtained in fixation in row
of ascending ethanol concentrations. All organs were visualized in great details without damaging the samples.
Conclusion:
New ethanol method is a great option for soft tissue fixation as well as the method for enhancing contrast among tissues
in examined organs.
matej.patzelt@centrum.czMatej Patzelt et al., OMICS J Radiol 2018, Volume 7
DOI: 10.4172/2167-7964-C1-022