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December 03-04, 2018 | Lisbon, Portugal
Public Health, Women's Health, Nursing and Hospital Management
Joint Event
Journal of Community Medicine & Health Education | ISSN : 2161-0711 | Volume 08
Rhinovirus infection is influenced by polymorphims in the immune
system related genes TOLLIP and IL6
R
hinovirus are picornavirus with over 150 serotypes and 3 species. Although usually
causing common colds, in Asthma, COPD and elders it may cause life-threatening
disease. Both environmental and genetic susceptibility factors may play a role in rhinovirus
epidemiology.
In the present study we evaluated the influence of immune-system related genetic variations
(TOLLIP rs5743899, IL6 rs1800795, IL1B rs16944, TNFA rs1800629) in the modulation
of rhinovirus infection suscetibility.
Blood samples and monthly nasal swabs were collected from 89 volunteers. DNA and
RNAwere purified with Qiagen column based kits. Viral RNAwas quantified by RTqPCR
on Lightcycler 1.1 (Roche). Polymorphisms were genotyped by PCR-RFLP.
TOLLIP and IL6 polymorphisms (but not IL1B and TNF) were found to influence
rhinovirus nasal detection. TOLLIP-G individuals were more often year-long rhinovirus
free (p<0.048). Additionally, if TOLLIP-G individuals were excluded from analysis,
non-IL6-C individuals showed higher rhinovirus titers (p<0.028). This fully agrees
with molecular mechanisms as free Rhinovirus particles, are detected by TLR2 which is
inhibited by TOLLIP. TOLLIP-G decreases TOLLIP expression, increasing TLR2 and
protecting from Rhinovirus infection. TOLLIP G also sustains production of IL6 needed
for viral clearance post-infection. However, in the absence of the TOLLIP G allele, and
of the IL6-C allele there is low IL6 production leading to a poorer Immune response and
higher viral titers.
31035@ufp.edu.ptAna Filipa Maia Rodrigues
Fernando Pessoa University, Portugal
Ana Filipa Maia Rodrigues et al., J Community Med Health Educ 2018, Volume:8
DOI: 10.4172/2161-0711-C7-051
Co-Authors
AM. Santos, R. Marino, AM. Ferreira,
ME. Barreira
and
JM. Cabeda
Fernando Pessoa University, Portugal