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December 03-04, 2018 | Lisbon, Portugal
Public Health, Women's Health, Nursing and Hospital Management
Joint Event
Journal of Community Medicine & Health Education | ISSN : 2161-0711 | Volume 08
Magdalena Kajdos
Polish Mother's Memorial
Hospital Research Institute
Poland
Magdalena Kajdos , J Community Med Health Educ 2018, Volume:8
DOI: 10.4172/2161-0711-C7-051
result. Median (as a measure of the average value in each group) and 1st and 3rd quartiles (as a
measure of the results spread) were counted for each type of test in sets (set 1, set 2 and set 3)
in the subgroups of patients. Comparisons between patients groups were made with the Mann-
Whitney test. The test hypothesis assumed that both analyzed samples (test and control) came
from the same population (or population with identical medians).
We compared each of the flow cytometry parameters between the control and endometriosis
groups to check which of the cytometry results differ the groups. We were looking for a
correlation between plasma and peritoneal fluid to see if there was a relationship between
the amount of microvesicles in these media. In plasma samples the statistically significant
differences were observed in three cases: set 3 VEGF + / MMP9 - higher percentage of
object marked by those antibodies in the control group, a larger percentage of microvesicles
with annexinV in the group with endometriosis and higher percentage of microvesicles with
MMP9 in the control group. It was also noticed that tests with annexin V plus another marker
(antibodies) gave few object to count whereas tests with VEGF plus another possible marker
gave much more objects to count. The same criteria were used to analyze samples of peritoneal
fluid. There was detected one significant difference between test and control group: set 3 VEGF
+ / MMP9 - higher percentage of objects in the control group. Like in plasma, microvesicles
marked by VEGF, also in combination with other markers, were the higher number of objects.
Moreover we observed higher percentage of objects marked by CK18 in peritoneal fluid in
both group.
There was a try to estimate the logistic regression to see if it was possible to predict, to which
of group patients belonged using microvesicles profile. The logistic regression models ware
developed for collected date of plasma and peritoneal fluid analysis. However the odds ratio
estimate was impossible due to the small number of samples.
Moreover we tried to cluster patients within the plasma and peritoneal fluid groups to visualize
the overall picture of the cytometry results between the groups. Clustering did not properly
separate two groups of patients. The results indicated the heterogeneity of the study group. The
heterogeneity was observed in both plasma and peritoneal fluid samples. The reason could be
clinical factors not included in the analyzed data.
Conclusion:
Results of the study did not confirm hypothesis that microvesicles with
proangiogenic factors (VEGF, MMP9) are produced in higher amount by endometriosis
foci. The research revealed presence of MVs in blood and peritoneal fluid samples in both
groups. The higher percentage of MVs with VEGF+/MMP9 and only MMP9 in blood was
unexpected result. Those factor are important in angiogenesis. Process, which is more advanced
in endometriosis foci, not in teratomas cysts. In peritoneal fluid analysis we also observed more
MVs VEGF+/MMP9 in control group. Moreover in peritoneal fluid there were noticed a lot of
microvesicles marked by CK18 in both grup. It was supsrising result, because cytokeratine 18
was choosen as e specific marker for endometrial cells.
To sum up, the study indicated single parametrs, which differing group of patients witch
endometriosis and control group (patients with teratomas). Most of the measurements did
not differentiate the analyzed groups of patients. The tests group was heterogeneity, while the
control group was quite small, which made difficulties to obtain statistically significant results.
The reason of heterogeneity in group of patient with endometriosis could be many. In this study
information of stage of endometriosis advanced and date of menstrual cycle in which was
samples collected were not compared with analyzed data. Perhaps these data had a significant
impact on variety results
magdalena.kajdos@gmail.com