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conferenceseries.com
March 22-23, 2017 | Rome, Italy
2nd World Congress on
Public Health & Nutrition
Volume 7, Issue 1 (Suppl) J Community Med Health Educ 2017
ISSN: 2161-0711, JCMHE an open access journal Public Health 2017
March 22-23, 2017
EFFECTS OF BARIATRIC SURGERY ON MYOCARDIAL RESISTANCE TO ISCHEMIA AND
REPERFUSION INJURY IN THE EXPERIMENT
Galina V Semikovaa, Elena E Davydovaa, Lucas G Carellia, Oxana Tkachuk a, Oleg V Kornyushin b, Alexander E Neumarkb, Iana G Toropovab and Alexander S
Polozovc
aFirst Pavlov State Medical University of Saint Petersburg, Russia
bFederal Almazov North-West Medical Research Centre, Russia
cPavlov Institute of Physiology- RAS, Russia
Statement of the Problem: Obesity, metabolic syndrome and diabetes mellitus type 2 are the most common pathological states in
the world. Although bariatric surgery is widely used to reduce the adverse effects caused by these states, its effect on cardiovascular
system is still not known. To solve this problem, studies on animals are needed to evaluate the effects of various bariatric procedures
on hemodynamic in normal and pathological states. The aim of this study is to conduct experiments on rats and to compare the
impact of various bariatric procedures on myocardial resistance to ischemia and reperfusion injury.
Methodology & Theoretical Orientation: The rats were subjected to surgeries for proximal stomach resection, ileal interposition
and laparotomy. 5-6 months after the surgeries, isolated hearts of the animals were perfused by Krebs-Henseleit solution in vitro
according to Langendorff heart. Coronary flow, systolic and diastolic blood pressure, heart rate and contractile function were
measured before ischemia period and during perfusion period. To identify areas of necrosis following ischemia, the hearts were
incubated with 2,3,5-triphenyltetrazolium chloride. The necrosis area was evaluated on digital photographs by manually contouring
the differentially colored left ventricle subsets.
Findings: 5-6 months after the surgeries, the proximal gastrectomy, but not the ileal interposition, contributed to the increase of
necrosis area in the heart after ischemia. Specific changes in the dynamics of coronary flow, systolic and diastolic blood pressure, heart
rate and contractile function during perfusion period were observed after the proximal stomach resection and the ileal interposition.
Conclusion & Significance: In normal conditions, the effects of bariatric procedures on myocardial resistance to ischemia and
reperfusion injury depend on the type of surgery. The data obtained are important to assess the impact of different bariatric procedures
on cardiovascular system and to develop the effective surgical approaches for the treatment of type II diabetes in patients with heart
disease.
J Community Med Health Educ 2017, 7:1 (Suppl)