physiotherapy-2016_posters-accepted-abstracts

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October 13-15, 2016 Kuala Lumpur, Malaysia

International Conference and Expo on

Physiotherapy

Volume 6, Issue 5 (Suppl)

J Nov Physiother

ISSN: 2165-7025 JNP, an open access journal

Physiotherapy 2016

October 13-15, 2016

J Nov Physiother 2016, 6:5 (Suppl)

Effect of cold plasma on the characteristics of DPPC liposomes

H Fahmy, A Hassan, G Abdelfatah, S Mohamed

and

M Gaber

Cairo University, Egypt

ecent progress in atmospheric plasmas has led to the creation of cold non-thermal atmospheric plasma (CAP). CAP is an ionized

gas that has tremendous applications in biomedical engineering and is used as a possible therapy in dentistry and oncology. The

aim of plasma interaction with tissue is not to denature the tissue, but rather to operate below the threshold of thermal damage and to

induce chemically specific response or modification. Liposomes are used as models for artificial cells. This report therefore investigates

the effect of cold plasma on 2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) liposomes prepared by thin film hydration method

which are used as a model for lipid bilayer membrane. DPPC liposomes were exposed to cold plasma 2, 3 and 5 minutes, respectively.

The effect of cold plasma on DPPC characterization parameters such as size, charge, FTIR absorption spectrum, UV spectrum and

phase transition temperature were investigated. The present study revealed that CAP could alter the molecular structure for DPPC

liposomes as depicted in the change in the FTIR absorption peaks at 3439 and 1687 cm-1. In addition, CAP affected the phase

transitions for the DPPC by shifting it to higher temperatures. Moreover, CAP led to the increase of DPPC liposome size. 2 min

exposure to CAP resulted in rapid coagulation of liposomes as depicted from the low zeta potential value obtained. However, the UV

absorption spectrum for DPPC liposomes was not altered by CAP exposure. Hence, this work highlighted that CAP may modify the

physical and chemical characteristics of DPPC liposomes.