pharmacovigilance-congress-2016_posters-accepted-abstracts

18 / 20

Page 61
conferenceseries.com
Volume 4, Issue 5 (Suppl) J Pharmacovigil 2016
ISSN:2329-6887 JP, an open access journal Pharmacovigilance Congress 2016
September 28-30, 2016
September 28-30, 2016 Toronto, Canada
6th Pharmacovigilance Congress
J Pharmacovigil 2016, 4:5 (Suppl)
Cytoprotective effect of Hypericum triquetrifolium turra. On cyclophosphamide–induced cardiotoxicity in
rat
Songul Cetik
Mardin Artuklu Üniversity
C ardiotoxicity is one of the limiting side effects of this commonly used anticancer agent and cardiotoxic effects of CP were found to
be dose-related cardiac damage, morphologically defined necrosis, bleeding. Hypericum triquetrifolium Turra.(HT) is a phenolic
component with antioxidative and anticarciogenic properties. This study aimed to investigate the possible protective effect of HT
on CP-induced cardiotoxicity. Serum aspartat transaminase (AST), alanine transaminase (ALT), lactate dehydrogenase (LDH),
malondialdehyde (MDA), creatine kinase-MB (CK-MB), total oxidant state (TOS), total antioxidant state (TAS), oxidative stress
index (OSI) was examined. Furthermore, the cardiac tissues were analysed histologically. Albino rats (Wistar, 3-4 months old, male,
weight 220 ± 20 g healthy) were randomly divided in 9 groups, each including seven animals: Group 1 (control) treated with 0,5ml
saline; groups 2 treated with 150 mg/kg CP, respectively; group 3, 4 and 5 treated with 25, 50 and 100 mg/kg HT; groups 6 , 7 and 8
treated with 25, 50 or 100 mg/kg HT+CP, group 9 treated with 0,5ml - %0,2 DMSO. The results was analyzed bye One Way Analysis
of Variance and Kruskal-Wallis One Way Analysis of Variance on Ranks Test. Levels of AST, ALT, LDH, MDA, CK-MB, TOS, OSI
were found high only in the CP groups. GSH and TAS levels were found low in the only CP groups. There was a dose-dependent
on the CP-induced cardiotoxicity. Hemorrhage, inflammatory cell infiltration and the separation of the muscle fibers in the heart
tissue supported the biochemical data. With 25, 50, 100 mg/kg HT, there was an important decrease in the CP toxicity and reduced
inflammation and lipid peroxidation in the heart tissue and increase of serum glutathione (GSH) and total antioxidant capacity (TAS)
levels were found when HT was applied. Based on these findings, it could be proposed that HT was a strong candidate in preventing
the CP-induced cardiotoxicity but further clinical studies should be done in order to verify its application on humans.
Healthcare providers’ knowledge and their counseling practice about warfarin in the university
teaching tertiary care hospital, addis ababa, ethiopia
Tamrat Assefa
Addis Ababa University, Ethiopia
Objective: The main objective was to assess healthcare providers’ knowledge about warfarin and their counseling practice on warfarin
at Tikur Anbessa Specialized Hospital (TASH).
Material and Methods: Cross sectional study design which included 164 pharmacists and physicians was conducted at TASH, Addis
Ababa Ethiopia. The assessment was through questionnaire which consisted of 15 multiple choice questions on warfarin knowledge
and 4 questions on counseling practice about warfarin (multiple choice and open ended questions).The data was analyzed with SPSS
and the differences between groups were compared using one-way ANOVA followed by turkey’s post-hoc test.
Results: Out of 15 questions the mean total score was 9.98 (SD=1.67).Among study participants, there was no one who gave correct
answers to all questions. The total score of the overall test were (9.45 + 1.63) by pharmacists, (10.06 + 1.49) by interns and (10.35
+ 1.77) by residents. In their counseling practice, among the total study participants, 61.6% and 29.3% of them responded as they
provide counseling services for all and only for new patients who are on warfarin respectively. Among the factors which have influence
on their counseling practice insufficient time and poor counseling environment predominantly affect 54.3% and 32.9% of study
participants respectively.
Conclusion: Based on this study finding it can be concluded that the knowledge of healthcare providers regarding oral anticoagulation
was inadequate. Residents had better knowledge than pharmacists and interns on questions related to warfarin. The study participants
had different experience on providing counseling for patients on warfarin.