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conferenceseries
.com
Volume 7, Issue 4 (Suppl)
Clin Exp Pharmacol
ISSN: 2161-1459 CPECR, an open access journal
Pharmacology Congress 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
8
th
World Congress on
Pharmacology and Toxicology
Testicular toxicity characters and mechanisms of hydroxyurea on rats
Han Yan, Li Zhou, Yong-wei Luo
and
Zu-yue Sun
Shanghai Institute of Planned Parenthood Research, China
Objective:
Apoptosis plays a dominant role in both spontaneous spermatogenesis and germ cell death. This study was aimed
to investigate the functions of related genes in testicular germ cell death induced by Hydroxyurea (HU).
Method:
Wild-type (WT) and FasL Transgenic (TG) DBA/C57BL mice were intraperitoneal injected with 400 mg/kg HU. 12
hours later, testes were collected. Histomorphology of testis was observed by stained with Periodic Acid Schiff (PAS). Apoptosis
was assessed by TUNEL assay. mRNA and protein levels of related genes were evaluated by quantitative RT-PCR and Western
blot respectively.
Results:
The 2×2 factorial design comparative experiments between WT and TG mice showed that the TG mice exhibited a
higher basal apoptotic index. The basal mRNA levels of Fas and FasL and protein levels of Fas, FasL, Caspase-3, Caspase-8 and
Caspase-9 in TG mice were also higher than that in WT mice. 12 hours after injection of HU, the testicular tubules exhibited
no significantly morphological changes but remarkably increased apoptosis index in both WT and TG mice, with the latter
having the higher amplitude. Although HU up-regulated the mRNA of apoptosis related genes such as Fas and FasL in both
TG and WT mice, the increased amplitude were more obvious in TG mice. By Western blot analysis, apoptosis related proteins
Fas, FasL Caspase-3, Caspase- 8 and Caspase-9 were significantly increased in both WT and TG mice, with TG mice exhibiting
a greater up-regulation.
Conclusion:
Germ cell apoptosis induced by HU treatment may be related to the FasL mediated signal transduction pathway.
Biography
Han Yan has completed the masters in Quality Assurance from National Evaluation Centre for the Toxicology of Fertility Regulating Drug. She has published
more than 20 papers in China and abroad reputed journals.
yanhan617617@126.comHan Yan et al., Clin Exp Pharmacol 2017, 7:4 (Suppl)
DOI: 10.4172/2161-1459-C1-020