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Volume 5 Issue 4(Suppl) Clin Pharmacol Biopharm
ISSN: 2167-065X CPB, an open access journal
Page 35
Pharma & Clinical Pharmacy Congress 2016
November 07-09, 2016
conferenceseries.com
November 07-09, 2016 Las Vegas, Nevada, USA
4th International
Pharma & Clinical Pharmacy Congress
Diallo Yacouba L, Clin Pharmacol Biopharm 2016, 5:4(Suppl)
Hemostasis property of Malian herbal plant used to manage bleeding event
Introduction: Bleeding diseases management is a big challenge in developing countries where diagnosis and drug access are
not easy. In Mali 80-90% of the population frequently used medicinal plant with a good response. Unfortunately, knowledge
on these plants is undocumented. Here, we investigated ten herbal plants currently used by traditional practitioners in Dioila
district (Mali) to treat bleeding conditions. e aim of this study was to investigate the coagulation properties of these plants
and identi ed the substance responsible for di erent hemostasis properties.
Materials & Methods: e hemostasis properties of water, ethanol and dichloromethane extracts from ten plants have
been investigated. e plants were selected a er ethnobotanical survey conducted in Dioila area in Mali. Fi een traditional
practitioners were interviewed in the survey and the ten plants currently used according to their high level of delity were
retained for this study. e e ect of the extracts on hemostasis parameters was investigated using whole blood from healthy
donor. All extracts were incubated with whole blood at the nal concentration of 0.25 g/L. Activating platelet time aPTT and
thrombin time were measured using coagulation automate (STA satellite®) at 0 and 30 min a er incubation. Bu er was used
as a control in the same condition. Results were expressed as ratio for aPTT and percentage for rombin time. All tests were
performed in double.
Results: We have investigated the e ect of twelve extracts from ten plants on aPTT and thrombin time at (0 and 30 min) a er
incubation. aPTT measurement directly a er incubation showed that eleven extracts gave a result lower than 1.2. Only extracts
from Pteleopsis myrtifolia bark and trunk, induced an aPTT beyond 1.2. A er 30 min incubation, aPTT value from all extracts
was lower than 1.2. In contrast, it seems that prothrombin time was not strongly modi ed by any extract.
Conclusion: Some extracts from herbal plants modi ed aPPT which could be associated to a hemostatic e ect. More
investigations are needed in order to con rm these ndings.
Biography
Diallo Yacouba L successively achieved his Medical study degree in 2000, the Hematology Special Training in Ivory Coast in 2008, Clinical Hemostasis and
Thrombosis Interuniversity Diploma in Lyon in 2009 and a Master’s degree in Vessel Biology, Physiology and Pathology from Paris University, France in 2010. His
research interest topic is hemostatis, especially in bleeding disease such us hemophilia. Due to the poor financial condition of the population in his country, he is
keenly interested to improve bleeding diseases treatment with medicinal plants. He is a member of many national and international scientist societies of Hematology
and Hemostasis.
Diallo Yacouba L
Hospital du Mali, Mali