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Volume 8
Journal of Gastrointestinal & Digestive System
ISSN: 2161-069X
Page 36
JOINT EVENT
Pediatric Gastro 2018
Digestive Diseases 2018
October 22-23, 2018
October 22-23, 2018 Berlin, Germany
3
rd
International Conference on
Digestive and Metabolic Diseases
Pediatric Gastroenterology Hepatology & Nutrition
13
th
International Conference on
&
Cysteinylated plasma albumin leads reduce antioxidant activity in nonalcoholic fatty liver disease
(NAFLD)
Abhishak C Gupta
Indian Institute of Technology, India
O
xidative stress is postulated to play an important role in liver disease progression. The degree of oxidized cysteine (Cys) 34
in human serum albumin (HSA) is correlated with oxidative stress related to pathological conditions and modulates its
physiological functions. We analyzed purified plasma albumin from 46 biopsy-proven NAFLD patients and 21 matched healthy
blood donors. The albumin modifications were analyzed by liquid chromatography coupled with electrospray ionization time-
of-flight mass spectrometer (ESI-TOF/MS). Relative % abundance of unmodified (intact) and modified isoforms of albumin
were compared between NAFLD and controls.
In vitro
ROS (reactive oxygen species) generation and antioxidant activity was
measured by mean fluorescence (MFI) of dihydrorhodamine (DHR) by flow cytometry in presence of purified albumin of
controls andNALFDpatients.Threemost prominent isoforms of albuminwere observed in the deconvoluted ESI spectrumwith
molecular masses of 66,438±2.8, 66,559±4.8 and 66,603±6 Da in controls and NAFLD patients represents intact, cysteinylated
and glycated isoforms of albumin respectively. Unmodified albumin was the predominant peak with 100% relative abundance
in healthy with calculated theoretical mass (66,438 Da, 542 aa). In contrast, the relative abundance of modified form with
addition of +119 Da (cysteinylation) of albumin was predominant (100%) in NAFLD. Cysteinylated isoform of albumin (cys-
Alb) was significantly higher in NAFLD patients than controls (100% v/s 52% - p<0.01). Circular dichroism (CD) spectrum
showed clear structural alterations in purified albumin from NAFLD patients as compared to controls. Further, albumin
antioxidant activity was measured by removal of ROS productions
in vitro
. Significant differences were observed in mean
fluorescence intensity of DHR in presence of purified albumin from controls and patients (51.5±5.8% vs 60.3±13.8%, p<0.001)
showed reduced antioxidant activity of albumin in NAFLD patients. Our results clearly showed that sustained oxidative stress
and reduced antioxidant activity is reflected by high levels of cysteinylated albumin in NAFLD patients.
abhigupta78@gmail.comJ Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C7-083