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Volume 8
Journal of Gastrointestinal & Digestive System
ISSN: 2161-069X
Page 40
JOINT EVENT
Pediatric Gastro 2018
Digestive Diseases 2018
October 22-23, 2018
October 22-23, 2018 Berlin, Germany
3
rd
International Conference on
Digestive and Metabolic Diseases
Pediatric Gastroenterology Hepatology & Nutrition
13
th
International Conference on
&
Prevelance of occult HBV in chronic hepatitis C and cryptogenic hepatitis patients
Cakal B
Department of Microbiology and Clinical Microbiology, Istanbul University, Turkey
O
ccult Hepatitis B Virus (HBV) infection (OBI) is considered as the possible phase of the HBV natural history but the
molecular mechanisms and clinical impact and epidemiological aspect of OBİ remains unclear. We investigated the
prevalence of OBI and its clinical impact among patients with Hepatitis C virus (HCV) infection and with cryptogenic hepatitis.
This study protocol was approved by the ethics committee of İstanbul University İstanbul School of Medicine (No: 2015/1519).
This prospective cohort study included a total of 60 HBsAg-negative patients (27 patients with chronic HCV and 33 patients
with cryptogenic hepatitis) were enrolled in the Department of Gastroenterology, Istanbul Faculty of Medicine. Liver tissue
samples had been obtained by percutaneous needle liver biopsy and immediately frozen and stored at -80°C. Total nucleic acids
were extracted from frozen liver biopsies using QIAamp DNA Mini Kit (Qiagen) according to the manufacturer’s instructions.
OBI was defined as HBV DNA positivity in 2 or more different viral genomic regions by nested polymerase chain reaction PCR
using 4 sets of primers in preS-S (S), precore-core (C), Pol, and X viral regions of the HBV genome. Plasmid HBV DNA 4.1 kb
and liver biopsy samples obtained from patients with chronic HBV infection (positive control) were used. Statistical analyses
were evaluated using Mann-Whitney U test, Chi-square test and Kruskal Wallis tests. The baseline characteristics of patients
are presented in figure 1. The prevalence of OBI was 25.9% (7/26) with 27.3% (9/33), 26.7% (16/60) in patients anti-HCV (+),
cryptogenic hepatitis, and totaly respectively. There wasn’t any significant differences for prevelance of OBİ between patients
with chronic HCV infection and cryptogenic hepatitis (P=0.907). Patients with anti-HCV (+), OBİ (+) were older compared
with patients anti-HCV (+), OBİ (-), (P: 0.033). As it is expected that cryptogenic hepatitis patients had higher serum alkaline
phosphatase and gamma-glutamyltransferase level (P<0.05). Clinical signifance and role of OBI in patients with chronic HCV
infection is controversial. Accordingly, first results of the study with respect to prevelance of OBİ is correlated with endemicity
of Hepatitis B infection; moreover OBİ can be associated with liver injury rather than chronic HCV infection. Therefore, it
appears that host factors rather than viral factors are more responsible for OBI.
bulentcakal@yahoo.comJ Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C7-083