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Volume 8
Journal of Gastrointestinal & Digestive System
ISSN: 2161-069X
Page 53
JOINT EVENT
Pediatric Gastro 2018
Digestive Diseases 2018
October 22-23, 2018
October 22-23, 2018 Berlin, Germany
3
rd
International Conference on
Digestive and Metabolic Diseases
Pediatric Gastroenterology Hepatology & Nutrition
13
th
International Conference on
&
Study of histone 3 acetylation in patients with Crohn’s disease
Ovidiu Fratila
University of Oradea, Romania
T
here was very little study on understanding fibrotic intestinal pathology in patients with Crohn’s disease since most studies
focused mainly on inflammatory pathway. In murine colitis study, it had been showed that the elevation of transforming
growth factor (TGFβ) can lead to increased activation of fibroblast and increased secretion of extracellular matrix protein
(ECM). Epigenetic mechanisms involving histone modification is proven to play an important role in intestinal inflammation.
Increased in histone deacetylase activity (HDAC) was found in many inflammatory conditions such as arthritis and cancer.
There are numbers of HDACis (histone deacetylase inhibitors), which result in acetylation of cell, which is essential for gene
expression. Few recent studies showed that in murine model of inflammatory colitis, HDAC inhibitor can reduce the overall
inflammatory symptoms. The data is lacking in human and hence, this study was performed based on the hypothesis that
histone acetylation will be low in the mucosa overlying a stricture area of CD patients compared to a non-stricture area in
the same patients. The first aim was to measure and compare the level of histone acetylation in the stricture mucosal area of
CD patients compared to a non-stricture mucosal area. The second aim was to see whether or not HDACi can reduce the
expression of collagen gene of mRNA in the intestinal fibroblast. Hypothesis is that histone acetylation will be low in the
mucosa overlying a stricture area of CD patients compared to a non-stricture area in the same patients. The results supported
the hypothesis and were consistent with previous experiment done in murine studies. We are getting closer to achieve our goal
of understanding histone acetylation in the CD bowel and this could lead potentially to novel therapeutic strategies for IBD.
ovidiufr@yahoo.co.ukJ Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C7-083