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Volume 7, Issue 5 (Suppl)
J Clin Exp Pathol
ISSN: 2161-0681 JCEP, an open access journal
Pathology Congress 2017
November 13-14, 2017
NOVEMBER 13-14, 2017 OSAKA, JAPAN
14
th
Asia Pacific Pathology Congress
Correlative analysis of
hMLH1
and
hMSH2
with
APC
gene in sporadic colorectal cancer in young north
Indian patients
Kim Vaiphei and Vikas Gupta
Postgraduate Institute of Medical Education and Research, India
S
poradic Colorectal Cancer (CRC) in patients is increasing rapidly in Indian population and half of the patients are less
than 50 years of age. No comprehensive molecular study has been carried out to analysis the basis for the disease occurring
in younger individuals. The study investigated frequency of
hMLH1
and
hMSH2
genes and
hMLH1
and
hMSH2
proteins
expressions, their prognostic significance and correlated with the Adenomatosis Polyposis Coli (
APC
) gene mutational status
by DNA sequencing in young CRC patients. Protein expression and promoter methylation of
APC
,
hMLH1
and
hMSH2
and
Mismatch Repair genes (MMR) were analyzed by immunohistochemistry and Methylation-Specific PCR (MSP), respectively
and correlated with patient’s data. Of 100 CRC,
hMLH1
and
hMSH2
loss were observed in 18 and 12, reduced expressions
in 50 and 38, respectively, 5 failed to express. Promoter hyper-methylation for
hMLH1
was detected in 50 and
hMSH2
in 10.
Combination of methylation of
hMLH1
and
hMSH2
gene was observed in 8 tumors. Significant correlation was observed
between histological tumor grade, methylation status and
hMLH1
gene expression (p<0.05). Normal expression for
hMLH1
and
hMSH2
was observed in all un-methylated tumors. Promoter methylation of
hMLH1
and
hMSH2
failed to influence survival
and correlated with loss of protein.
APC
gene mutation was observed in 45% patients with no differential in distribution. Our
observations suggest inactivation of MMR gene via hyper-methylation lead to functional loss resulting in tumor aggressiveness
and role of
APC
gene appeared not to play a major role in tumor progression in these young patients.
Biography
Kim Vaiphei is currently serving as a Professor at Department of Histopathology, Post Graduate Institute of Medical Education and Research, Chandigarh, India.
She has received MBBS in 1983 and MD Pathology in 1987. She is a Fellow of International Union against Cancer (UICC-WHO), National Academy of Medical
Sciences India and Indian College of Pathology. She holds various levels of faculty positions in the Department of Histopathology, PGIMER and remained as
Professor since 2006. Her area of research includes molecular pathways of cancer development. She has more than 300 publications and attended more than 50
national and more than 20 international conferences.
kvaiphei2009@gmail.comKim Vaiphei et al., J Clin Exp Pathol 2017, 7:5 (Suppl)
DOI: 10.4172/2161-0681-C1-040