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Volume 7, Issue 5 (Suppl) J Clin Exp Pathol
ISSN: 2161-0681 JCEP, an open access journal Pathology Congress 2017
November 13-14, 2017
NOVEMBER 13-14, 2017 OSAKA, JAPAN
14th Asia Pacific Pathology Congress
Synaptic Adhesion Like Molecule (SALM4) regulates angiogenic functions via VEGFR2 activation
Dong Young Kim
Yonsei University, Republic of Korea
S ynaptic Adhesion Like Molecules (SALMs) is the adhesion molecules, highly enriched in nervous system, include five
members (SALM 1-5). All SALMs promote neurite outgrowth, while SALM4 uniquely increases the number of primary
processes extending from the cell body. However, the property of SALM4 in Endothelial Cell (EC) is still unknown. Here, we
discovered that SALM4 mRNA expression was increased during differentiation from Endothelial Progenitor Cell (EPC) to
EC. Unlike other SALMs, SALM4 was expressed specifically in EC. To find functions of SALM4 , we performed in vitro assays.
Wound and chemotactic migration assays showed that knock down of SALM4 attenuates EC migration. Next, we found tube
formation was decreased tube length in SALM4 deletion EC. EC Survival was reduced in SALM4 depletion. In mouse organs,
SALM4 shows organ specificity, it was mainly expressed brain and kidney. Consistent with this observation, EC recruitment
impaired in SALM4 KO mice injected matrigel with VEGF. Aortic sprouting reduced in SALM4 KO mice aorta implanted
matrigel. To elucidate the mechanism of SALM4 under VEGF treatment, we analyzed VEGFR2 activation. Silencing SALM4
in EC suppressed phosphorylation of VEGFR2. Moreover, downstream of VEGFR2 signaling was also reduced. These results
suggest that SALM4 has a potential role in regulating EC migration via activation of VEGFR2.
Biography
Dong Young Kim is currently pursuing his Doctor’s course in Vascular Biology at Yonsei University, Republic of Korea. He has completed his Bachelor’s degree in Biochemistry
at Yonsei University. His research fields are tumor angiogenesis and identifying angiogenic functions of endothelial cell enriched genes in endothelial cells.
Dong Young Kim, J Clin Exp Pathol 2017, 7:5 (Suppl)
DOI: 10.4172/2161-0681-C1-041