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Parkinsons 2016
December 05-07, 2016
Volume 6 Issue 6(Suppl) J Alzheimers Dis Parkinsonism
ISSN: 2161-0460 JADP, an open access journal
conferenceseries .com
December 05-07, 2016 Phoenix, USA
2nd International Conference on
Parkinson’s Disease & Movement Disorders
Jes Paul, J Alzheimers Dis Parkinsonism 2016, 6:6(Suppl)
Dopamine D1 and D 2 receptor subtypes functional regulation in unilateral rotenone lesioned
Parkinson’s rat model: Effect of serotonin, dopamine and norepinephrine
Jes Paul
American University of St Vincent, Saint Vincent and The Grenadines
Introduction: Parkinson’s disease (PD) is due to widespread degeneration in the central and peripheral nervous systems.
The hallmark pathology remains in the dopaminergic striatal insufficiency and degeneration of dopaminergic neurons in the
substantia nigra (SN).
Objectives: The present study analyzed the effect of serotonin (5-HT), dopamine and norepinephrine (NE) as treatment on
rotenone induced hemi-Parkinson’s disease in rats and its role in the regulation of Dopamine receptor subtypes in the Corpus
Straiatum (CS) of the experimental rats.
Methods: Unilateral stereotaxic single dose infusions of rotenone were administered to the substantia nigra of adult male
Wistar rats. Neurotransmitters –serotonin (5-HT), dopamine and norepinephrine (NE) treatments were given to rotenone
induced hemi-Parkinson’s rats. Dopamine receptor and its subtypes (D1 and D2) binding assay were done. Gene expression
studies of Dopamine D1 and D2 were done using real-time PCR.
Results: Scatchard analysis of Dopamine and Dopamine D2 receptor showed a significant increase (p<0.001) and Dopamine
D1 receptor showed a significant decrease (p<0.001) in the Bmax in Corpus Striatum of the PD rats compared to control. These
altered parameters were reversed to near control in the serotonin and norepinephrine treated Parkinson’s disease rats and no
change was observed in Dopamine treated Parkinson’s disease rats. Real-time PCR results confirmed the receptor data.
Conclusion: Our results showed serotonin and norepinephrine functionally reversed in Dopamine receptors in rotenone
induced hemi-Parkinson’s rat. This has clinical significance in the therapeutic management of Parkinson’s disease.
Biography
Jes Paul have done Ph.D. in Neurology & Molecular Cell Biology, stem cells with 3 years’ post-doctoral training, Master’s degree with 7 years’ post-graduate training
and Good Laboratory Practice (GLP).My publications include 15(Research gate, Pub med) Papers in international journals (Pub Med) and Presented 16 Abstracts
in various international conferences and he is also an Editorial member of IRPH Journal since four years. Currently he is working as a Research Associate (Albany
medical centre cardiovascular science).