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Volume 7, Issue 5 (Suppl) Otolaryngol
ISSN: 2161-119X Otolaryngol, an open access journal Otolaryngology 2017
October 18-19, 2017
October 18-19, 2017 Dubai, UAE
4th International Conference on
Rhinology and Otology
Effect of PepsinA on Heat Shock Protein 70 (Hsp70) response in patients with chronic rhinosinusitis
Wang Jing
Sichuan University, China
The objectives of this study are to investigate the relation between laryngopharyngeal re ux and chronic rhinosinusitis
(CRS) and to explore the e ect of pepsinA on the level of Hsp70. Nasal tissue specimens, nasal secretions and blood plasma
obtained from 23 CRS patients with nasal polyps (CRSwNP), 26 CRS patients without nasal polyps (CRSsNP) and 9 normal
controls were studied using Enzyme-Linked Immunosorbent Assay (ELISA) to measure pepsin levels in nasal secretions and
blood plasma, Western analysis to measure Hsp70 and pepsinA levels in nasal tissue specimens and Quantitative real-time
Polymerase Chain Reaction (Q-PCR) to detect the expression of pepsinogenA, HSPA5 and PTGS2 in nasal tissue specimens.
e expression of PepsinA in nasal secretions was signi cantly higher in patients with CRS than in normal controls (p<0.05).
Hsp70 expression level was signi cantly increased in pepsinA-positive turbinate mucosa, compared with controls (P<0.001)
and pepsinA-negative turbinate mucosa in CRSwNP and CRSsNP patients (P<.001). Similarly, the Hsp70 expression level
was signi cantly increased in pepsinA-positive polyp tissues, compared with the controls (p=0.021) and pepsinA-negative
polyp tissues in CRSwNP patients (p=0.016). ere was no signi cant Hsp70 expression di erence between pepsinA-negative
turbinate mucosa or polyp tissues and controls. Furthermore, no association was found between the presence of pepsinA and
HSPA5 in nasal tissue specimens. e results suggest that the LPR may play a role in the development of CRS through the re ux
of pepsinA. Increased expression of Hsp70 may be associated with the pathogenic mechanism of pepsinA.
Biography
Wang Jing is currently majoring in Clinical Medicine, working toward her MD from West China Medical School, Sichuan University, China. Her research direction
is the relationship between Chronic rhinosinusitis and Laryngopharyngeal reflux. She is also interested in Nasal Physiology. She has published 2 SCI paper and
has participated in international conferences.
Wang Jing, Otolaryngol 2017, 7:5 (Suppl)
DOI: 10.4172/2161-119X-C1-022