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Volume 4, Issue 4 (Suppl) J Laser Opt Photonics, an open access journal
ISSN: 2469-410X Optics 2017
November 15-17, 2017
November 15-17, 2017 | Las Vegas, USA
8th International Conference and Exhibition on
Lasers, Optics & Photonics
Photon crystal–supported surface electromagnetic waves: A tool to study dynamics of receptor-ligand
interactions with living bacteria and cells and to launch ultralong propagating surface plasmons
S K Sekatskii
Laboratoire de Physique de la Matière Vivante, Switzerland
We present a label-free biosensor based on registration of the photonic crystal (PC)-supported surface waves. s-polarized surface
wave is used to detect changes in the thickness of an adsorbed layer, while p-polarized surface wave provides independent
data on the liquid refraction index thus enabling the segregation of surface and volume effects. With this method we achieve mass
sensitivity at the level of 0.3 pg/mm2 and refraction index (RI) sensitivity at the level of 10-7 RIU/Hz1/2. Other characteristic feature
of this biosensor is large, of the order of 1 micron, surface wave penetration depth into an external media, which enables to study
intermolecular interactions not only at (a few) monolayers level, but also for such large objects as bacteria, cell organells and even
certain cells. We elaborated a chitosan-based protocol of surface modification of the sensor chip enabling to produce sufficiently dense
and homogeneous (mono) layers of live E. coli bacteria and then these layers have been exploited as a generic “immobilized receptor
layer” to study for the first time kinetics of adsorption of different ligands onto their (i.e. living bacteria’s) surface. Other applications
of our approach are the use of specially prepared PC with thin (8 nm) metal layers to support ultralong plasmon propagation in
Pd (for ultrasensitive hydrogen detection) and Co (for magnetoplasmonics) and in Au in blue and UV spectral ranges. (Note that
in all these cases this is meaningless to speak about plasmons without PC: the plasmon propagation length is just of the order of
wavelength).
Biography
S K Sekatskii has completed his PhD from Institute of Spectroscopy, Russian Academy of Sciences, Moscow. Currently, he is working as a Senior Researcher of Ecole
Polytechnique Fédérale de Lausanne, Switzerland (a permanent position). He has published around 150 papers in reputed journals.
S K Sekatskii, J Laser Opt Photonics 2017, 4:4 (Suppl)
DOI: 10.4172/2469-410X-C1-017