obesity-diet-2018-scientifictracks

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Journal of Obesity & Weight Loss Therapy | Volume: 08

August 28-29, 2018 | Paris, France

Obesity, Diet and Nutrition

International Conference on

Roberta Dourado

Cavalcante da Cunha de Sá

Federal University of São Paulo,

Brazil

The efforts dedicated to finding the cure for obesity and associated disorders

lead to an intense interest in adipocyte metabolism. The consumption of ω-3

fatty acids (FA) presents beneficial effects on changes caused by obesity. The aim

of this study was to investigate the adipokines secretion of isolated adipocytes

from obese mice induced by high fat (HF) diet, supplemented or not with fish

oil (FO) [rich in ω-3 FA (EPA/DHA, 5:1)] with emphasis on the differential

response of subcutaneous and visceral adipose deposits, inguinal (ING) and

retroperitoneal (RP) regions, respectively. C57BL/6J mice received control (CO)

or HF diet for eight weeks. Supplementation with FO (2 g/Kg p.c., 3 times/week)

was initiated eight weeks after the induction of obesity, remaining until the end;

totaling 16 weeks of experimental protocol. The white adipose tissue ING and

RP were removed for isolation of adipocytes that were subjected to D'MEM/10%

FBS culture for 30 hours. At the end, adipokines concentrations in the culture

supernatant were determined using specific ELISA kits. The adipocytes of the HF

group showed a significant hypertrophy followed by an increase in the secretion of

proinflammatory cytokines TNF-α and IL-6 compared to the CO group, whereas

the HF+FO group presented total reversion of this effect, in both ING and RP

adipocytes. There was no difference in secretion of adiponectin. The relevance

of isolated adipocytes in the secretion of these cytokines is highlighted here. The

adipocytes are affected by the HF diet and the FO has a protective effect on these

parameters.

Effects of treatment with fish oil on subcutaneous and visceral adipocytes dysfunction triggered by obesity

in mice

co-authors:

Cruz M M, Batini F, Barbosa N, Farias

T S M

and

Alonso Vale M I C

Federal University of São Paulo, Brazil

Biography

Roberta Dourado Cavalcante da Cunha de Sá has completed her PhD from Federal University of São Paulo. She is a Pharmacist, Master of Science and has

published one paper as first author and three papers as co-author.

Roberta Dourado Cavalcante da Cunha de Sá et al., J Obes Weight Loss Ther 2018, Volume: 08

DOI: 10.4172/2165-7904-C5-069