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Journal of Obesity & Weight Loss Therapy | Volume: 08
August 28-29, 2018 | Paris, France
Obesity, Diet and Nutrition
17
th
International Conference on
Maysa Mariana Cruz et al., J Obes Weight Loss Ther 2018, Volume: 08
DOI: 10.4172/2165-7904-C5-069
Maysa Mariana Cruz
Federal University of São Paulo,
Brazil
W
hite adipose tissue (WAT) is specialized in storing energy in the form of
triacylglycerols, providing fatty acids (FA) according to the energy demand
of the individual, being expanded in obesity. Obesity is associated with the onset
of metabolic syndrome, which in turn correlates with WAT dysfunction that
includes changes in mitochondrial, metabolic and adipogenic genes expression
in adipocytes. We recently demonstrated that palmitoleic acid (C16:1n7), a
monounsaturated FA, increases the metabolic and oxidative capacity of 3T3-L1
adipocytes, modifying some bioenergetic parameters related tomitochondrial and
metabolic functions. However, there are no data about its effects on the expression
of WAT obesogenic genes from animals submitted to obesity by a high fat diet
(HFD). Thus, in the present work, male C57BL/6 mice were submitted to the
control diet (CO) or HFD for eight weeks. From the 5th week, the animals received
C16:1n7 (300 mg/kg/day) or water for 30 days, by gavage. After euthanasia, the
inguinal WAT was removed for analysis of ATGL, HSL, Perilipin, LPL, FABP4,
Lipin, GLUT-4, Adiponectin, Leptin, CEBP-alpha and PPAR-gamma by real time
RT-PCR gene expression. The following genes showed increased expression in the
HFD group: ATGL, HSL, LPL, Lipin, GLUT-4, Leptin, PPAR-gamma and CEBP-
alpha, all of which were partially or completely reversed by the C16:1n7 treatment.
FABP4 expression was positively modulated in both the HFD and HFD+C16:1n7
groups, as well as the adiponectin. Accordingly, we suggest that palmitoleic acid is
modulating and/or adapting the mice WAT helping them to deal with the energy
demand from HFD. Thus, the animals treated with palmitoleic acid respond
differently from the obese group, and therefore, the imposed condition of obesity
by HFD.
Biography
Maysa Mariana Cruz is pursuing her PhD from Federal University of São Paulo. She is a Pharmacist. She has completed her Master of Science and has published
one paper as first author and seven papers as coauthor.
maysamariana@gmail.comEffects of palmitoleic acid on the expression of obesogenic genes in adipose tissue of mice submitted to a
high fat diet
co-authors:
Farias T M, De Sá R D C, Batini F,
Barbosa N
and
Alonso-Vale M I C
Federal University of São Paulo, Brazil