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conferenceseries.com
October 20-22, 2016 Rome, Italy
11th International Conference and Expo on
Nanoscience and Molecular Nanotechnology
Volume 7, Issue 5 (Suppl) J Nanomed Nanotechnol 2016
ISSN: 2157-7439 JNMNT an open access journal NanoScience 2016
October 20-22, 2016
Covalent immobilization of candida rugosa lipase on a novel enzymatic nanocomposite membrane
prepared by covalent attachment of magnetic nanoparticle to poly acrylonitrile membrane
Marzieh Aghababaie, Masoud Beheshti, Amir Razmjou and Abdol-Khalegh Bordbar
University of Isfahan, Iran
Enzymatic nanocomposite membranes which are the combination of nanocomposite membranes and immobilized enzymes
have received increasing attention, recently. In this study, poly acrylonitrile ultrafiltration membrane was aminated and then
magnetic nanoparticles which were modified by glutaraldehyde or cyanuric chloride were covalently attached on the surface of
membrane. Afterwards, Candida Rugosa Lipase (CRL) was covalently immobilized on this nanocomposite membrane. Nanoparticles
and nanocomposite membrane were characterized with various techniques such as SEM, TEM, XRD, FTIR, ATR, AFM, contact
angle goniometry and surface free energy measurement. The evidence of immobilization was also done by ATR, enzyme activity
and loading efficiency. It was found that the PAN nanocomposite membrane increased the relative activity and loading capacity in
comparison to UF membrane. The Km and vmax values represents the increasing of substrate affinity and decreasing of catalytic
activity of immobilized enzyme due to the mass transfer limitation for both nanocomposite membranes. Thermal, storage, and
operational stability of immobilized enzyme increased significantly which make it a suitable candidate for to bio-catalytic processes.
Biography
Marzieh Aghababie is a PhD student at University of Isfahan. She is working on her PhD thesis entitled, “Developing an enzymatic nanocomposite membrane
bioreactor using immobilized lipase on magnetic nanoparticle for the production of biodiesel”. In her Master’s, she published three papers in “Journal of Food
Engineering”, “Food and Bioproducts Processing Journal” and “Nutrition and Food Sciences Research”. Last year, she gave an oral presentation about enzymatic
nanocomposite membranes in the international conference on membrane science and technology and published a paper in this regard in “Food and Bioproducts
Processing Journal”.
Marzieh Aghababaie et al., J Nanomed Nanotechnol 2016, 7:5 (Suppl)