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Clinical Microbiology: Open Access | ISSN: 2327-5073 | Volume: 7
Microbiology: Education, R&D and Market
7
th
Annual Summit on
September 28-29, 2018 | San Antonio, USA
Interference of
Bacillus cereus
and
Clostridium perfringens
isolation from frozen buffalo meat with 16S
rRNA Sequencing of
B. cereus
isolate conducted in India
Ujjal Sen
and
Anil Mahadeo Garode
Kalinga University, India
I
ndia produces 1.49 million tons of buffalo meat contributing 45.56% of world buffalo meat production.
Bacillus cereus
and
Clostridium perfringens
both are spore formers. Hence, tolerate high temperature, high pressure, radiation etc. Consequently,
germinate in favorable condition.
B. cereus
is aerobic but,
C. perfringens
anaerobic in nature. Both spoil and contaminate frozen buffalo
meat product during processing in an abattoir.
B. cereus
causes a series of illness like nausea, vomiting, diarrheal syndrome, emetic
syndrome, abdominal pain and produces lethal enterotoxin.
C. perfringens
attributed to protein enterotoxin, during sporulation can
infect the wound, gas gangrene, intense abdominal cramps, gas, and diarrhea (nausea and vomiting are rare). Meat contaminated
with
C. perfringens
leads to approximately 160,000 disease cases annually in the Netherlands. Vacuum packing contains CO
2
and N
2
to inhibit the spore formers. But as
B. cereus
is aerobic so if there is low in said gases so
B. cereus
will reappear. In this research, out
of 61 frozen meat sample, 52 positive pink color colonies with lecithinase halo zone around the colony on MYP agar and 9 negatives
for B. cereus. This article also reveals, among 26 samples of frozen meat all are positive for
C. perfringens
appear as yellowish - gray or
black colonies with rotten smell of egg and lecithinase activity on perfringens agar supplemented with TSC or SFP undergo anaerobic
condition. Microbiological risk assessment analysis of abattoir air reveals out of 25 samples, 51% are positive for B. cereus, 25 samples
of water are 100% free from B. cereus, out of 25 swab samples of slaughter equipment 60% are positive for
B. cereus
and out of 25 swab
samples of food handlers are negative. One isolate from positive
B. cereus
colonies isolated and subjected to 16S rRNA sequencing
analysis which chromatogram and blast results identified as Bacillus spp. EC2 16S ribosomal RNA gene, partial sequence. Above all,
the risk and hazards obtained more with
C. perfringens
as food spoilage contaminant, but, the association of
B. cereus
with meat also
prevail equal risk for human in this study. Hence, Microbiological quality monitoring, implementation of international standards,
safe production practice of meat may reduce the risk of food born disease.
ujjal_sen@hotmail.comClin Microbiol 2018, Volume: 7
DOI: 10.4172/2327-5073-C3-040