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Clinical Microbiology: Open Access | ISSN: 2327-5073 | Volume: 7
Microbiology: Education, R&D and Market
7
th
Annual Summit on
September 28-29, 2018 | San Antonio, USA
Molecular characterization of cotton infecting
Begomoviru
s
(es)
and DNA satellites associated with cotton
leaf curl disease in Pakistan
Malik Nawaz Shuja
1, 2
and
Muhammad Tahir
1
1
National University of Sciences and Technology, Pakistan
2
Kohat University of Science and Technology, Pakistan
C
otton leaf curl disease (CLCuD) has been a considerable encumbrance to the cotton production worldwide including Pakistan.
The disease has been caused by a complex of
Begomoviruses
in association with cotton leaf curl Multan
alpha
/
beta-satellite
.
Previously, we reported a new strain of cotton infecting
Begomovirus
from District Layyah, a probable recombinant of Cotton leaf
curl
Kokhran virus
and Cotton leaf curl
Multan virus
– the two predominant species attributed to CLCuD in Pakistan. In this study,
symptomatic cotton samples were collected from the cotton belt in Punjab province. Total nucleic acid from leaf tissues was extracted
and subjected to PCR. Thirty full-length genomic components were cloned and sequenced, corresponding to
Begomovirus
and
associated DNA
alpha
-and
beta-satellite
. Further, a partial dimeric (0.63 mer) DNA-A, (0.55 mer) beta- and (0.9 mer)
alpha
satellite
clones were constructed in a binary vector and used in Agrobacterium-mediated inoculation of
Nicotiana benthamiana, N. tabacum,
Lycopersicon esculentum, Cucurbita
and
Cucumis sativus
. Leaf curling, deformation and yellowing like symptoms were observed on
inoculated plants. Following PCR amplification, the viral DNA, as well as sub-genomic components, were detected in newly emerging
non-inoculated leaves. Both molecular and biological studies indicate that this new isolate is a virulent strain of CLCuD and has the
potential to aggravate symptomology.
maliknshuja@yahoo.comClin Microbiol 2018, Volume: 7
DOI: 10.4172/2327-5073-C3-040