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Volume 6, Issue 1 (Suppl) Clin Microbiol
ISSN: 2327-5073 CMO, an open access journal Microbiology & Mycotoxins 2017
February 27-28, 2017
February 27-28, 2017 Amsterdam, Netherlands
7th Euro Global Summit on
Clinical Microbiology and Mycotoxins
Early proteomic changes induced by fusariotoxin single and combined exposures on human hepatocytes
Marie-Caroline Smith
Laboratoire Universitaire de Biodiversité et Ecologie Microbienne, France
While the reality of mycotoxin co-contamination of food commodities is nowwell-established, the assessment of the toxicological
impact of mycotoxin mixtures is still rare. Moreover, studies concerning the mechanistic cellular response to mycotoxins
(alone or in mixture) are lacking. Among the infinite number of possible mycotoxin mixtures found, combinations of toxins from
Fusarium spp. (called fusariotoxins) are particularly widespread in the North temperate zone of the world and therefore of interest.
In this context, our main objective was to compare the cellular mechanisms involved in response to single and combined exposures
of the human hepatocyte cell line HepaRG to two relevant fusariotoxins, deoxynivalenol and zearalenone. After 1 h of exposure
with deoxynivalenol and/or zearalenone at low cytotoxic doses (IC10), proteomes of HepaRG cells were analyzed by LC-MS/MS
and compared to the control condition without mycotoxin. Among the 3000 identified proteins per sample, 55 showed a significant
enhanced or reduced abundance compared to the non-exposed cells. Interestingly, none of these 55 proteins were in common
between the cells exposed to deoxynivalenol and those exposed to zearalenone. Noteworthy, very few proteins were common between
the mixture and the toxins alone. Cells exposed to deoxynivalenol showed an increased expression of proteins involved in DNA
topological changes, chromosome segregation and proteolysis, whereas zearalenone mainly induced changes for proteins involved in
response to steroid hormone stimulus. Concerning the mixture, the main affected biological processes were, among others, cell cycle
phase, DNA packaging and cell division. Thus, these results highlighted different cellular pathways responded to different single and
combined mycotoxins exposures.
Biography
Marie-Caroline Smith completed her Engineering Degree in Biochemistry and she is currently pursuing PhD at University of Brest, France. She is working on “The
impact of fusariotoxins co-exposure on human cells”. More specifically, she is studying the cellular mechanisms of the human monocytic cell line THP-1 as well
as the human hepatic cell line HepaRG, involved in the response to the exposure of one or more Fusarium mycotoxins in acute and chronic exposure conditions
through toxicology and proteomic approaches. She recently published a review article entitled ‘Natural co-occurrence of mycotoxins in foods and feeds and their
in vitro combined toxicological effects’.
Marie-Caroline Smith, Clin Microbiol 2017, 6:1(Suppl)