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Volume 6, Issue 1 (Suppl) Clin Microbiol
ISSN: 2327-5073 CMO, an open access journal Microbiology & Mycotoxins 2017
February 27-28, 2017
February 27-28, 2017 Amsterdam, Netherlands
7th Euro Global Summit on
Clinical Microbiology and Mycotoxins
Stability of masked mycotoxins in the human gut
Silvia W Gratz1, T Yoshinari2 and S MacDonald3
1University of Aberdeen, UK
2Division of Microbiology, National Institute of Health Sciences, Japan
3Fera Science Ltd, York, UK
C ereal grains are commonly contaminated with a range of mycotoxins and their plant-derived masked metabolites. These
conjugated metabolites are present in food and their contribution to toxicity either directly or indirectly through release of
the parent mycotoxins is unknown. This study aims to assess the fate of common masked mycotoxins under conditions prevailing
in the gut. The work assesses the metabolism and transport of glucoside metabolites of common trichothecenes and zearalenone
compounds in the human gut in vitro. All masked mycotoxins were stable under conditions prevailing in the upper GI tract and were
not absorbed intact through epithelial monolayers. Unabsorbed mycotoxins are likely to be delivered to the colon where they will be
subject to microbial activity. We found that human gut microbiota efficiently hydrolyzed all masked mycotoxins. Trichothecenes were
fully released as parent mycotoxins whereas zearalenone compounds were fully hydrolyzed and then further metabolized to unknown
metabolites. Our results demonstrate that masked mycotoxins will reach the colon intact to be released as parent mycotoxins by
gut microbiota and are therefore, contribute to mycotoxin exposure in humans. Furthermore, masked zearalenone compounds are
metabolized by gut microbiota and the identity and toxicity of metabolites are yet unknown.
Biography
Silvia W Gratz holds an MSc in Human Nutrition (University Vienna, Austria) and a PhD in Food Toxicology (University of Kuopio, Finland). She has been working
as a Research Fellow at Rowett Institute of Nutrition and Health since 2007. Her research work focuses on “The role of diet in gut health, intestinal toxicity and gut
microbiology”. She has published 15 original articles as well as four reviews and three book chapters and acts as Editorial Board Member of Frontiers in Predictive
Toxicology.
Silvia W Gratz et al., Clin Microbiol 2017, 6:1(Suppl)