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Volume 7, Issue 6 (Suppl) J Bacteriol Parasito
ISSN: 2155-9597 JBP, an open access journal Microbiology 2016
November 28-29, 2016
November 28-29, 2016 Valencia, Spain
7th World Congress on
Microbiology
Non-induction of resistance amongst Salmonella Typhi strain passaged through sub lethal dose of Beri
honey
Mahrukh Ghafoor, Muhammad Amir Zaheer, Usman Qamar, Sidra Saleem and Abdul Hannan
University of Health Sciences, Pakistan
Introduction &Aim: Resistance to conventional anti-typhoid drugs is well documented and the recent emergence of fluoroquinolone
resistance has made it very difficult and expensive to treat typhoid fever. As the therapeutic approach becomes even more limited, it
is crucial to probe into non-conventional modalities. In this perspective, honey is a promising nominee for skirmishing antimicrobial
resistance. Antimicrobial activity of honey is well established against variety of bacteria. It contains a broad range of antibacterial
compounds that act synergistically at multiple sites, thus possibly making bacterial resistance unlikely. In this study, the ability of
honey to resist the occurrence of resistance under conditions that induce resistance in antibiotics was analyzed.
Methods: Minimum inhibitory concentrations (MICs) of two antibacterials, Ciprofloxacin and Beri honey were determined against
Salmonella Typhi using broth dilution technique. The isolates thus obtained were then exposed and passaged through serially
increasing the sub lethal concentrations of the two in test tubes, till the concentrations reached the original minimum inhibitory
concentrations. After successive passages, the MICs of both bacterial inhibitors against Salmonella Typhi were determined again using
broth dilution technique.
Results:After exposure to the sub lethal dose, the MIC of Ciprofloxacin against Salmonella Typhi rose up to eight times. The minimum
inhibitory concentration of honey remained unchanged from its original value even after exposure to sub lethal doses.
Conclusion: The emergence of extensive resistance to antibiotics has arguably occurred due to their misuse and overuse but also
as a natural phenomenon. The results in this study show that sub lethal concentrations of the extraordinary natural antimicrobial
agent, Beri honey, do not favor the development of resistant bacterial phenotype. Here we demonstrate that Salmonella Typhi did
not develop resistance against honey, as it did against Ciprofloxacin. Further exploration of the molecular and cellular basis of this
behavior is the next line of research.
J Bacteriol Parasitol 2016, 7:6 (Suppl)