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conferenceseries.com
Volume 7, Issue 6 (Suppl) J Bacteriol Parasito
ISSN: 2155-9597 JBP, an open access journal Microbiology 2016
November 28-29, 2016
November 28-29, 2016 Valencia, Spain
7th World Congress on
Microbiology
Isolation and virtual screening of antimicrobial prodigiosin pigment from oxalotrophic Serratia
marcescens OX_R strain
Samadhan Ramchandra Waghmode1 and Mangesh V Suryavanshi2
1Elphinstone College, India
2National Centre for Cell Science, India
Prodigiosin is a multifaceted secondary metabolite produced by Serratia spp. having great potential as a pharmaceutical agent. In
the present study we demonstrate that oxalate supplementation in peptone glycerol production media increased organoleptic
characters and yield of prodigiosin pigment extracted from oxalotrophic Serratia marcescens OX_R isolated from Indian bat guano
sample. The pigment was demonstrated in vitro as an antibacterial agent against common opportunistic skin surface pathogen
Staphylococcus aureus NCIM 5021 strain as killing activity by agar well diffusion method. The docking analysis and pharmacophore
modeling indicated that the probable mechanism of action of the prodigiosin was against Staphylococcus aureus DNA gyrase protein.
The pigment was also found to efficiently dye both cotton and latex polymer. In summary, we describe here an oxalotrophic Serratia
marcescens which may serve as a potent and economical resource of prodigiosin which owing to its dyeing and anti-bacterial activities
finds future avenues to be developed as dressing material for nosocomial subjects or burn victim patients.
Quinolone resistant molecular mechanisms in Escherichia coli isolated from University Hospital in
Egypt
Sherine Ahmed Abd El-Rahman Aly
Assiut University, Egypt
Fluorpquinolone resistant E. coli (FQREC) is an important cause of many infectious diseases worldwide. Our goal is to detect the
prevalence of FQREC in Egypt in addition to unrevealing the most important molecular mechanisms of FQ resistance. Forty
E. coli isolates were tested for their ciprofloxacin MIC by E-test and for mutations in genes coding for DNA Gyrase (gyrA & gyrB),
Topoisomerase IV (parC & parE) and transcriptional regulators of AcrAB efflux pump; soxR, soxS, marR and acrR using Polymerase
Chain Reaction (PCR) and sequencing. The contribution of PMQR to resistance was based on PCR detection of qnrA, qnrB, qnrS,
qnrD, aac(6’)-lb-cr, and qepA determinants. All FQREC isolate had at least double mutations within gyrA plus a third mutation in
parC. Some FQR isolates also manifested an additional mutation either in the parC or in parE. Regarding the AcrAB pump regulators,
stop mutations both in soxR and acrR were recorded in addition to silent mutations in marA. Interestingly the A12S mutation in
soxS discovered in canine E. coli isolates have been found for the first time in 2 of human isolates of this study. Three of the FQSEC
showed decreased susceptibility to ciprofloxacin, one of them harbor a single gyrA mutation and another one showed a stop codon
in soxR. qnrS and aac(6’lb) were the most prominent plasmid found in 13 and 14 FQREC isolates respectively. qnrA. qnrB and qepA
were found in fewer isolates. Interestingly, one FQREC isolate harbor all the five plasmids together. Two of the FQSEC isolates with
reduced susceptibility contain PMQR.
J Bacteriol Parasitol 2016, 7:6 (Suppl)