influenza-2016_posters-accepted-abstracts

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Volume 4, Issue 6(Suppl) J Infect Dis Ther
ISSN: 2332-0877 JIDT, an open access journal
Page 73
Influenza 2016
September 12-13, 2016
conferenceseries.com
Influenza
September 12-13, 2016 Berlin, Germany
2nd International Conference on
J Infect Dis Ther 2016, 4:6(Suppl)
Influenza vaccination of medical residents and nurses at the three major teaching hospitals in Iran: across-
sectional survey
Zahra Khazaeipour and Neda Ranjbarnovin
Tehran University of Medical Sciences, Iran
Objectives: Health care workers (HCWs) who are unimmunized against influenza put through patients to unnecessary risk of
infection.
Objectives: The aim of this study was to evaluate the vaccination status of HCWs, knowledge of and attitudes towards influenza
vaccination.
Study design: A cross-sectional survey.
Methods: This cross-sectional study was performed in 459 HCWs of three hospitals between October 2006 and February 2007. They
received a username and password to enter in the ” website and filled self-administered online questionnaire.
Results: The influenza vaccination coverage for the 2006-2007 seasons was 14.4% (range, 11.2% to17.6%). In logistic regression
model of variables, only taking the vaccine in the future year (OR=2.44, CI 95%: 1.21-4.89) was significantly associated with influenza
vaccination uptake. The mean knowledge score of residents were 24.0±4.4 (range, 9-34) and nurses were 24.1±4.9 (range, 10-33;
P=0.9). Resident and nurses who taking the vaccine in the future year, residents who recommend the vaccine to coworkers or family
and nurses who having children less than 16 years at home had significantly higher knowledge scores (P-value<0.0001).
Conclusion: Our data showed that influenza vaccination coverage is low. We will need education and communication strategies to
overcome the lack of knowledge and interest.
The neuraminidase universal epitope of influenza A virus induces a protective immune response in mice
Zirak Javanmard Mahdieh1, Taghizadeh Morteza2, Moghaddam Pour Masoud2 and Arasteh Javad1
1Islamic Azad University of Tehran, Iran
2Razi Vaccine and Serum Research Institute, Iran
Human infection with influenza A virus is associated with a high mortality and morbidity and causes worldwide pandemics.
There is essential to improve a universal vaccine against influenza pandemic. We identified a total of 12 conserved epitopes in viral
neuraminidase proteins containing human T-cell epitopes for N1 & N2 subtypes. In this study, we use the epitope-based vaccine
designed by immunoinformatics tools to predict the binding of B-cell and T-cell epitopes (class I and class II human leukocyte
antigens [HLA]). BCPREDS was used to predict the B-cell epitopes. Propred, Propred I, netMHCpan and netMHCIIpan were used
to predict the T-cell epitopes. The 3D molecular model was constructed by Swiss Model server and N-Glycosylation sites excluded
from estimated regions. Important parameters like antigenicity and hydropathicity analyzed by Protean program. This sequence
was cloned into the prokaryotic expression vector Pet-41b(+). BALB/c mice were immunized with different dosages of recombinant
protein and the immune responses were determined in the form of protective response against influenza virus, antibodies titers, spleen
cells lymphocyte proliferation and the levels of interferon-γ and interleukin-4 cytokines. We observed an increase in the number of
influenza virus-specific IFNγ-secreting splenocytes, composed of populations marked by CD4+ and CD8+ T cells producing IFNγ or
TNFα. Upon challenge with influenza virus, the vaccinated mice exhibited decreased viral load in the lungs and a delay in mortality.
T-cells recognizing conserved epitopes were significant contributor to decreasing viral load and controlling disease severity during
heterosubtypic infection in animal models.