influenza-2016_posters-accepted-abstracts

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Volume 4, Issue 6(Suppl) J Infect Dis Ther
ISSN: 2332-0877 JIDT, an open access journal
Page 70
Influenza 2016
September 12-13, 2016
conferenceseries.com
Influenza
September 12-13, 2016 Berlin, Germany
2nd International Conference on
J Infect Dis Ther 2016, 4:6(Suppl)
Evaluation of immunogenic properties recombinant fusion protein 4xM2e-HA influenzaAvirus expressed
in MDCK cell line
Morteza Taghizadeh1, 2, Shamsi Shahrabadi M1, Moghaddampour M1, 2 and Tebianian M2
1Iran University of Medical Sciences, Iran
2Razi Vaccine and Serum Research Institute, Iran
Background&Aim:The recent pandemic swineH1N1 influenza (2009) outbreak demonstrated that egg-based vaccinemanufacturing
does not adequately respond to pandemic strains. Recent study has established an alternative for subunit vaccine by the use of the
recombinant. We try produced universal vaccine 4M2e-HA that can be produced in large scale in reasonable time.
Methods: In this study a recombinant 4xM2e-HA gene of influenza A virus was designed and expressed in MDCK cell which could
be secreted out of cells. Immunized mice with this protein induced both humoral and cellular response against influenza A virus.
Result: The immunized mice showed increased immunological indicators such as IFN-γ and IL-2, IL-12, IL-4 and induced suitable
CTL response, also antibody against fusion protein can be neutralized both heterologous and homologues influenza virus.
Conclusion: These findings suggest that 4xM2e-rHA expression in MDCK cell may provide a new approach for developing a novel
universal vaccine that may protect not only specifically against a new circulating strains but is expected to protect broadly against
new virus strains possessing common epitopes with conserved sequences. The 4xM2e-rHA protein is a highly purified single protein
that might enhance tolerance against the antigen and allows administration of higher doses and produce stronger immunological
response and protection against the mentioned virus.
Comparison betweenMDCK andMDCK-SIAT1 cell lines as preferred host for cell culture-based influenza
vaccine production
Parvaneh Mehrbod1,Asghar Abdoli 1, Hoorieh Soleimanjahi2, Abbas Jamali1, Shima Gholami1, Zahra Kianmehr 3, Neda Feizi 1, Maryam Saleh 1, Fariborz Bahrami1,
Talat Mokhtari-Azad4, Mohsen Abdoli1 and Masoumeh Tavassoti Kheiri1 3
1Pasteur Institute of Iran, Iran
2Tarbiat Modares University, Iran
3Shahed University, Iran
4Tehran University of Medical Sciences, Iran
Increasing demands for seasonal influenza vaccine and the need for faster methods of vaccine production during flu pandemics
and the threat posed by highly pathogenic avian influenza viruses, have made cell culture a suitable substrate for influenza vaccine
manufacturers. Cell-adapted viruses replicate with high fidelity, which are expected to have potent vaccine immunogenicity. The
aim of this study was evaluating MDCK and MDCK-SIAT1 cell lines for their ability to produce the yield of influenza virus. Yields
obtained for influenza virus H1N1 grown in MDCK-SIAT1 cell was almost the same level as MDCK; however, H3N2 virus grown
in MDCK-SIAT1 showed lower peak of viral titers in comparison with MDCK cells. The optimized MOI to infect the cells on plates
and microcarrier was 0.01 and 0.1 for H1N1 and 0.001 and 0.01 for H3N2, respectively. MDCK-SIAT1 cells have the capacity to be
considered as an alternative mean to manufacture cell-based flu vaccine, especially for the human strains (H1N1), due to its antigenic
stability and high titer of influenza virus production compared to egg inoculation.