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Volume 5, Issue 3 (Suppl)
J Infect Dis Ther, an open access journal
ISSN:2332-0877
Infectious Diseases 2017
August 21-23, 2017
3
rd
Annual Congress on
Infectious Diseases
August 21-23, 2017 San Francisco, USA
Microbiota in healthy Saudi males with various degrees of obesity
Steve Harakeh
1
, Suhad Bahijri
2
, Ghada Ajabnoor
2
, Ahmed Al-Hejin
3
, Suha Farraj
1
, Salah Birnawi
1
and
Esam Azhar
1
1
King Abdulaziz University, Saudi Arabia
2
Saudi Diabetes Research Group, Saudi Arabia
Background:
Obesity is amajor modern global problem that is facing humanity. It is associated withmajor health complications
and is a risk factor for diabetes and cardiovascular diseases (CVD). There is an alarming rate of increase in obesity among the
Saudi population, placing a huge burden on health and economic resources in the country. Gut microbiota (GM) has been
recently reported to be involved in the pathogenesis of many metabolic disorders and diseases including obesity, diabetes, and
CVD.
Objective:
The objective of this study was to identify obesity-associated GM dysbiosis and their relationship to body mass
index (BMI) among healthy Saudi males with various degrees of obesity.
Methodology:
A total of 48 healthy males with different degrees of obesity were recruited for this study. All those filled
out a questionnaire related to their nutritional habits, health conditions and demographics. Their height, body weight, hip
and waist circumference were measured (BMI and age). Stool samples were collected and genomic DNA was extracted from
those samples. The DNA samples were sequenced via 16s rRNA next generation sequencing (MiSeq), sequencing reads were
trimmed, analyzed and filtered and assigned to taxonomic units.
Results:
The results indicated the presence of various bacteriological groups in the GM of individuals with different degrees
of obesity. There were 37 species (found in small numbers) which were found only in the underweight, 46 in the normal,
17 in the overweight and 31 in the obese. However, at the family level the following were found: Desulfovibrio, prevotella
and mesorhizobium in the underweight in the obese Lactobacilli and
Loktanella
. In the normal most
Bifidobacterium
and
Leuconostoc
and finally in the overweight the most present is
Nesternkonia
.
Conclusion/Recommendations:
The results indicated that a variety of bacterial strains and microbiota populations were
present among our study participants. Final goal is to use the data obtained from this study as biomarkers for early prediction,
progress and for treating obesity in Saud Arabia.
sharakeh@hotmail.comJ Infect Dis Ther 2017, 5:3 (Suppl)
DOI: 10.4172/2332-0877-C1-027