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conferenceseries
.com
Volume 5, Issue 3 (Suppl)
J Infect Dis Ther, an open access journal
ISSN:2332-0877
Infectious Diseases 2017
August 21-23, 2017
3
rd
Annual Congress on
Infectious Diseases
August 21-23, 2017 San Francisco, USA
Detection of the
mcr
-1 colistin resistance gene and extended-
spectrum beta-lactamase (ESBL)-producing
Escherichia coli
from
poultry in Qatar
Nahla Eltai
Qatar
A
ntimicrobial resistance (AMR) is a growing public health concern worldwide and is one of the top health challenges
facing humanity in the 21
st
century. AMR among Enterobacteriaceae is rapidly increasing especially to third-generation
cephalosporins and carbapenems. Further, strains carrying mobilized colistin resistance (
mcr
) genes 1 and 2 have been isolated
from humans, food-producing animals, and environment. Uncontrolled use of antibiotics in animals in large scale could
be one of the major contributing factors to generation and spread of antibiotic resistance. No studies have been done to
evaluate antimicrobial resistance in animals in Qatar. This study aimed at establishing a primary baseline data for prevalence
of antimicrobial resistance among food animals in Qatar. 172 fecal samples were obtained from two broiler farms and one
live bird market in Qatar and 90
Escherichia coli
(
E. coli
) bacteria were isolated and subjected to antimicrobial susceptibility
testing using E-test method. 90% (81/90) of the isolates were resistant to at least one of the 16 tested antibiotics. 15.5%
(14/90) of the isolates were colistin resistant, 2.2% (2/90) were extended spectrum β lactamase (ESBL) producers and similar
percentages were multi-drug resistant (MDR) to four antibiotic classes. ESBL-producing
E. coli
and colistin resistant isolates
were confirmed using double disc susceptibility testing and PCR, respectively. In summary, our results indicate that high
antimicrobial resistance in food producing animals in Qatar, including ESBL and colistin resistance. Such AMR bacteria could
be easily transmitted to humans through consumption of undercooked food or noncompliance with hygiene practices, which
mandates prompt development and implementation of stewardship program to control and monitor the use of antimicrobial
agents in community and agriculture.
nahla.eltai@qu.qu.eduJ Infect Dis Ther 2017, 5:3 (Suppl)
DOI: 10.4172/2332-0877-C1-027