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conferenceseries
.com
Volume 5, Issue 3 (Suppl)
J Infect Dis Ther, an open access journal
ISSN:2332-0877
Infectious Diseases 2017
August 21-23, 2017
3
rd
Annual Congress on
Infectious Diseases
August 21-23, 2017 San Francisco, USA
Impact of
L119F-GSTe2
DDT/pyrethroid resistance mutation on the fitness cost and malaria transmission
of Anopheles funestus in Cameroon
Mu-Chun Chiang
Liverpool School of Tropical Medicine, UK
Objective:
Insecticide resistance inmosquitoes is potentially threatening the control of malaria. However, the ability of resistant
mosquitoes to transmit malaria may be altered by reduced fitness associated with insecticide resistance (IR) genes. This study
explored the fitness costs associated with
L119F-GSTe2
(a single gene mutation which confers resistance against DDT) in an
An. funestus
population in Cameroon.
Methodology:
Mosquito collections were carried out in Obout, Cameroon. Bioassays were performed on reared F1
An.
funestus
and cone assays were performed on 5 commercial nets. Molecular analysis included PCR species identification of
An.
funestus
from extracted gDNA and TaqMan assays for Plasmodium infection and
GSTe2-L119F
gene mutation. Oviposition
rate was recorded for 100 F0
An. funestus
but could not be compared between resistant and susceptible mosquitoes (with and
without
GSTe2-L119F
mutation respectively) due to unforeseen circumstances.
Results:
The Plasmodium infection rate in the Obout
An. funestus
(20% for
P. falciparum
) was significantly higher than
previously recorded in the north of Cameroon. Bioassays demonstrated resistance against all classes of insecticides except
for the organophosphates. Of the nets tested in the cone assay, only the top of PermaNet® 3.0 demonstrated full efficacy and
suspiciously, Olyset® net showed complete loss of efficacy.
Conclusion:
This study has unfortunately had setbacks due to problems with chemical reagents. Nevertheless, this is the
first study to characterize the resistance profile of
An. Funestus
mosquitoes in Cameroon. The collected
An. funestus
were
confirmed as a major Plasmodium vector and based on the bioassay results, organophosphates should be encouraged for use
in indoor residual spraying. Tighter inspection and examination of nets sold in Cameroon is required as Olyset® net that is
available in local pharmacies was suspected to be forged.
muchunchiang@hotmail.comJ Infect Dis Ther 2017, 5:3 (Suppl)
DOI: 10.4172/2332-0877-C1-027